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A novel cloning strategy for one-step assembly of multiplex CRISPR vectors

One key advantage of the CRISPR/Cas9 system in comparison with other gene editing approaches lies in its potential for multiplexing. Here, we describe an elaborate procedure that allows the assembly of multiple gRNA expression cassettes into a vector of choice within a single step, termed ASAP(Adapt...

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Detalles Bibliográficos
Autores principales:	Zuckermann, Marc, Hlevnjak, Mario, Yazdanparast, Haniyeh, Zapatka, Marc, Jones, David T. W., Lichter, Peter, Gronych, Jan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269432/ https://www.ncbi.nlm.nih.gov/pubmed/30504793 http://dx.doi.org/10.1038/s41598-018-35727-3

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