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Sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis

Both sorbitol and sucrose are synthesized in source leaves and transported to fruit for supporting fruit growth in tree fruit species of the Rosaceae family. In apple (Malus domestica), antisense suppression of aldose-6-phosphate reductase, the key enzyme for sorbitol synthesis, significantly decrea...

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Autores principales: Li, Mingjun, Li, Pengmin, Ma, Fengwang, Dandekar, Abhaya M., Cheng, Lailiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269491/
https://www.ncbi.nlm.nih.gov/pubmed/30510767
http://dx.doi.org/10.1038/s41438-018-0064-8
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author Li, Mingjun
Li, Pengmin
Ma, Fengwang
Dandekar, Abhaya M.
Cheng, Lailiang
author_facet Li, Mingjun
Li, Pengmin
Ma, Fengwang
Dandekar, Abhaya M.
Cheng, Lailiang
author_sort Li, Mingjun
collection PubMed
description Both sorbitol and sucrose are synthesized in source leaves and transported to fruit for supporting fruit growth in tree fruit species of the Rosaceae family. In apple (Malus domestica), antisense suppression of aldose-6-phosphate reductase, the key enzyme for sorbitol synthesis, significantly decreased the sorbitol concentration but increased the sucrose concentration in leaves, leading to a lower sorbitol but a higher sucrose supply to fruit in these plants. In response to this altered carbon supply, the transgenic fruit had lower concentration of sorbitol and much higher concentration of glucose but similar levels of fructose, sucrose, and starch throughout fruit development relative to the untransformed control. Activities of sorbitol dehydrogenase, fructokinase, and sucrose phosphate synthase were lower, whereas activities of neutral invertase, sucrose synthase, and hexokinase were higher in the transgenic fruit during fruit development. Transcript levels of MdSOT1, MdSDHs, MdFK2, and MdSPS3/6 were downregulated, whereas transcript levels of MdSUC1/4, MdSUSY1-3, MdNIV1/3, MdHKs, and MdTMT1 were upregulated in the transgenic fruit. These findings suggest that the Sucrose cycle and the sugar transport system are very effective in maintaining the level of fructose and provide insights into the roles of sorbitol and sucrose in regulating sugar metabolism and accumulation in sorbitol-synthesizing species.
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spelling pubmed-62694912018-12-03 Sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis Li, Mingjun Li, Pengmin Ma, Fengwang Dandekar, Abhaya M. Cheng, Lailiang Hortic Res Article Both sorbitol and sucrose are synthesized in source leaves and transported to fruit for supporting fruit growth in tree fruit species of the Rosaceae family. In apple (Malus domestica), antisense suppression of aldose-6-phosphate reductase, the key enzyme for sorbitol synthesis, significantly decreased the sorbitol concentration but increased the sucrose concentration in leaves, leading to a lower sorbitol but a higher sucrose supply to fruit in these plants. In response to this altered carbon supply, the transgenic fruit had lower concentration of sorbitol and much higher concentration of glucose but similar levels of fructose, sucrose, and starch throughout fruit development relative to the untransformed control. Activities of sorbitol dehydrogenase, fructokinase, and sucrose phosphate synthase were lower, whereas activities of neutral invertase, sucrose synthase, and hexokinase were higher in the transgenic fruit during fruit development. Transcript levels of MdSOT1, MdSDHs, MdFK2, and MdSPS3/6 were downregulated, whereas transcript levels of MdSUC1/4, MdSUSY1-3, MdNIV1/3, MdHKs, and MdTMT1 were upregulated in the transgenic fruit. These findings suggest that the Sucrose cycle and the sugar transport system are very effective in maintaining the level of fructose and provide insights into the roles of sorbitol and sucrose in regulating sugar metabolism and accumulation in sorbitol-synthesizing species. Nature Publishing Group UK 2018-12-01 /pmc/articles/PMC6269491/ /pubmed/30510767 http://dx.doi.org/10.1038/s41438-018-0064-8 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Li, Mingjun
Li, Pengmin
Ma, Fengwang
Dandekar, Abhaya M.
Cheng, Lailiang
Sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis
title Sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis
title_full Sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis
title_fullStr Sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis
title_full_unstemmed Sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis
title_short Sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis
title_sort sugar metabolism and accumulation in the fruit of transgenic apple trees with decreased sorbitol synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269491/
https://www.ncbi.nlm.nih.gov/pubmed/30510767
http://dx.doi.org/10.1038/s41438-018-0064-8
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