Real-time assembly of ribonucleoprotein complexes on nascent RNA transcripts

Cellular protein-RNA complexes assemble on nascent transcripts, but methods to observe transcription and protein binding in real time and at physiological concentrations are not available. Here, we report a single-molecule approach based on zero-mode waveguides that simultaneously tracks transcripti...

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Detalles Bibliográficos
Autores principales: Duss, Olivier, Stepanyuk, Galina A., Grot, Annette, O’Leary, Seán E., Puglisi, Joseph D., Williamson, James R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269517/
https://www.ncbi.nlm.nih.gov/pubmed/30504830
http://dx.doi.org/10.1038/s41467-018-07423-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269517/
https://www.ncbi.nlm.nih.gov/pubmed/30504830
http://dx.doi.org/10.1038/s41467-018-07423-3

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