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Discrimination of Multi-Origin Chinese Herbal Medicines Using Gas Chromatography-Mass Spectrometry-Based Fatty Acid Profiling

Multi-origin Chinese herbal medicines, with herbs originating from more than one species of plants, is a common phenomenon but an important issue in Traditional Chinese Medicines (TCMs). In the present study, a gas chromatography-mass spectrometry (GC-MS)—based fatty acid profiling approach to rapid...

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Autores principales: Zhang, Xiao-Jing, Qiu, Jian-Feng, Guo, Lan-Ping, Wang, Ying, Li, Peng, Yang, Feng-Qing, Su, Huanxing, Wan, Jian-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269696/
https://www.ncbi.nlm.nih.gov/pubmed/24335614
http://dx.doi.org/10.3390/molecules181215329
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author Zhang, Xiao-Jing
Qiu, Jian-Feng
Guo, Lan-Ping
Wang, Ying
Li, Peng
Yang, Feng-Qing
Su, Huanxing
Wan, Jian-Bo
author_facet Zhang, Xiao-Jing
Qiu, Jian-Feng
Guo, Lan-Ping
Wang, Ying
Li, Peng
Yang, Feng-Qing
Su, Huanxing
Wan, Jian-Bo
author_sort Zhang, Xiao-Jing
collection PubMed
description Multi-origin Chinese herbal medicines, with herbs originating from more than one species of plants, is a common phenomenon but an important issue in Traditional Chinese Medicines (TCMs). In the present study, a gas chromatography-mass spectrometry (GC-MS)—based fatty acid profiling approach to rapidly discriminate multi-origin Chinese medicines in terms of species and medicinal parts was proposed and validated using tuberous roots (Curcumae Radix) and rhizomes (Curcumae Rhizoma and Curcumae Longae Rhizoma) derived from four Curcuma species (e.g., C. wenyujin, C. kwangsiensis, C. phaeocaulis and C. longa) as models. Both type and content of fatty acids varied among different species of either tuberous roots or rhizomes, indicating each species has its own fatty acid pattern. Orthogonal partial least squares discriminant analysis (OPLS-DA) and hierarchical clustering analysis (HCA) based on dataset of global fatty acid profiling showed that either tuberous roots or rhizomes samples could be clearly classified into four clusters according to their species. Furthermore, those tested samples could also be discriminated in terms of their medicinal parts (e.g., tuberous root and rhizome). Our findings suggest that the proposed GC-MS-based fatty acid profiling followed by multivariate statistical analysis provides a reliable platform to discriminate multi-origin Chinese herbal medicines according to species and medicinal parts, which will be helpful for ensuring their quality, safety and efficacy.
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spelling pubmed-62696962018-12-20 Discrimination of Multi-Origin Chinese Herbal Medicines Using Gas Chromatography-Mass Spectrometry-Based Fatty Acid Profiling Zhang, Xiao-Jing Qiu, Jian-Feng Guo, Lan-Ping Wang, Ying Li, Peng Yang, Feng-Qing Su, Huanxing Wan, Jian-Bo Molecules Article Multi-origin Chinese herbal medicines, with herbs originating from more than one species of plants, is a common phenomenon but an important issue in Traditional Chinese Medicines (TCMs). In the present study, a gas chromatography-mass spectrometry (GC-MS)—based fatty acid profiling approach to rapidly discriminate multi-origin Chinese medicines in terms of species and medicinal parts was proposed and validated using tuberous roots (Curcumae Radix) and rhizomes (Curcumae Rhizoma and Curcumae Longae Rhizoma) derived from four Curcuma species (e.g., C. wenyujin, C. kwangsiensis, C. phaeocaulis and C. longa) as models. Both type and content of fatty acids varied among different species of either tuberous roots or rhizomes, indicating each species has its own fatty acid pattern. Orthogonal partial least squares discriminant analysis (OPLS-DA) and hierarchical clustering analysis (HCA) based on dataset of global fatty acid profiling showed that either tuberous roots or rhizomes samples could be clearly classified into four clusters according to their species. Furthermore, those tested samples could also be discriminated in terms of their medicinal parts (e.g., tuberous root and rhizome). Our findings suggest that the proposed GC-MS-based fatty acid profiling followed by multivariate statistical analysis provides a reliable platform to discriminate multi-origin Chinese herbal medicines according to species and medicinal parts, which will be helpful for ensuring their quality, safety and efficacy. MDPI 2013-12-11 /pmc/articles/PMC6269696/ /pubmed/24335614 http://dx.doi.org/10.3390/molecules181215329 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhang, Xiao-Jing
Qiu, Jian-Feng
Guo, Lan-Ping
Wang, Ying
Li, Peng
Yang, Feng-Qing
Su, Huanxing
Wan, Jian-Bo
Discrimination of Multi-Origin Chinese Herbal Medicines Using Gas Chromatography-Mass Spectrometry-Based Fatty Acid Profiling
title Discrimination of Multi-Origin Chinese Herbal Medicines Using Gas Chromatography-Mass Spectrometry-Based Fatty Acid Profiling
title_full Discrimination of Multi-Origin Chinese Herbal Medicines Using Gas Chromatography-Mass Spectrometry-Based Fatty Acid Profiling
title_fullStr Discrimination of Multi-Origin Chinese Herbal Medicines Using Gas Chromatography-Mass Spectrometry-Based Fatty Acid Profiling
title_full_unstemmed Discrimination of Multi-Origin Chinese Herbal Medicines Using Gas Chromatography-Mass Spectrometry-Based Fatty Acid Profiling
title_short Discrimination of Multi-Origin Chinese Herbal Medicines Using Gas Chromatography-Mass Spectrometry-Based Fatty Acid Profiling
title_sort discrimination of multi-origin chinese herbal medicines using gas chromatography-mass spectrometry-based fatty acid profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269696/
https://www.ncbi.nlm.nih.gov/pubmed/24335614
http://dx.doi.org/10.3390/molecules181215329
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