Cargando…

Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study

In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized...

Descripción completa

Detalles Bibliográficos
Autores principales: Souza-Moreira, Tatiana M., Severi, Juliana A., Lee, Keunsook, Preechasuth, Kanya, Santos, Emerson, Gow, Neil A. R., Munro, Carol A., Vilegas, Wagner, Pietro, Rosemeire C. L. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269720/
https://www.ncbi.nlm.nih.gov/pubmed/23839114
http://dx.doi.org/10.3390/molecules18078095
_version_ 1783376533290745856
author Souza-Moreira, Tatiana M.
Severi, Juliana A.
Lee, Keunsook
Preechasuth, Kanya
Santos, Emerson
Gow, Neil A. R.
Munro, Carol A.
Vilegas, Wagner
Pietro, Rosemeire C. L. R.
author_facet Souza-Moreira, Tatiana M.
Severi, Juliana A.
Lee, Keunsook
Preechasuth, Kanya
Santos, Emerson
Gow, Neil A. R.
Munro, Carol A.
Vilegas, Wagner
Pietro, Rosemeire C. L. R.
author_sort Souza-Moreira, Tatiana M.
collection PubMed
description In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level.
format Online
Article
Text
id pubmed-6269720
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62697202018-12-17 Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study Souza-Moreira, Tatiana M. Severi, Juliana A. Lee, Keunsook Preechasuth, Kanya Santos, Emerson Gow, Neil A. R. Munro, Carol A. Vilegas, Wagner Pietro, Rosemeire C. L. R. Molecules Article In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. MDPI 2013-07-09 /pmc/articles/PMC6269720/ /pubmed/23839114 http://dx.doi.org/10.3390/molecules18078095 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Souza-Moreira, Tatiana M.
Severi, Juliana A.
Lee, Keunsook
Preechasuth, Kanya
Santos, Emerson
Gow, Neil A. R.
Munro, Carol A.
Vilegas, Wagner
Pietro, Rosemeire C. L. R.
Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study
title Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study
title_full Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study
title_fullStr Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study
title_full_unstemmed Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study
title_short Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study
title_sort anti-candida targets and cytotoxicity of casuarinin isolated from plinia cauliflora leaves in a bioactivity-guided study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269720/
https://www.ncbi.nlm.nih.gov/pubmed/23839114
http://dx.doi.org/10.3390/molecules18078095
work_keys_str_mv AT souzamoreiratatianam anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy
AT severijulianaa anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy
AT leekeunsook anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy
AT preechasuthkanya anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy
AT santosemerson anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy
AT gowneilar anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy
AT munrocarola anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy
AT vilegaswagner anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy
AT pietrorosemeireclr anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy