Cargando…
Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study
In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269720/ https://www.ncbi.nlm.nih.gov/pubmed/23839114 http://dx.doi.org/10.3390/molecules18078095 |
_version_ | 1783376533290745856 |
---|---|
author | Souza-Moreira, Tatiana M. Severi, Juliana A. Lee, Keunsook Preechasuth, Kanya Santos, Emerson Gow, Neil A. R. Munro, Carol A. Vilegas, Wagner Pietro, Rosemeire C. L. R. |
author_facet | Souza-Moreira, Tatiana M. Severi, Juliana A. Lee, Keunsook Preechasuth, Kanya Santos, Emerson Gow, Neil A. R. Munro, Carol A. Vilegas, Wagner Pietro, Rosemeire C. L. R. |
author_sort | Souza-Moreira, Tatiana M. |
collection | PubMed |
description | In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. |
format | Online Article Text |
id | pubmed-6269720 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62697202018-12-17 Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study Souza-Moreira, Tatiana M. Severi, Juliana A. Lee, Keunsook Preechasuth, Kanya Santos, Emerson Gow, Neil A. R. Munro, Carol A. Vilegas, Wagner Pietro, Rosemeire C. L. R. Molecules Article In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. MDPI 2013-07-09 /pmc/articles/PMC6269720/ /pubmed/23839114 http://dx.doi.org/10.3390/molecules18078095 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Souza-Moreira, Tatiana M. Severi, Juliana A. Lee, Keunsook Preechasuth, Kanya Santos, Emerson Gow, Neil A. R. Munro, Carol A. Vilegas, Wagner Pietro, Rosemeire C. L. R. Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
title | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
title_full | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
title_fullStr | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
title_full_unstemmed | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
title_short | Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study |
title_sort | anti-candida targets and cytotoxicity of casuarinin isolated from plinia cauliflora leaves in a bioactivity-guided study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269720/ https://www.ncbi.nlm.nih.gov/pubmed/23839114 http://dx.doi.org/10.3390/molecules18078095 |
work_keys_str_mv | AT souzamoreiratatianam anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT severijulianaa anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT leekeunsook anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT preechasuthkanya anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT santosemerson anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT gowneilar anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT munrocarola anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT vilegaswagner anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy AT pietrorosemeireclr anticandidatargetsandcytotoxicityofcasuarininisolatedfrompliniacaulifloraleavesinabioactivityguidedstudy |