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Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures

Tanshinones are abietane-type norditerpenoid quinone natural products found in a well-known traditional Chinese medicinal herb, Salvia miltiorrhiza Bunge. The copalyl diphosphate synthase of S. miltiorrhiza (SmCPS) is the key enzyme in the first step for transformation of geranylgeranyl diphosphate...

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Autores principales: Cheng, Qiqing, He, Yunfei, Li, Geng, Liu, Yujia, Gao, Wei, Huang, Luqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269745/
https://www.ncbi.nlm.nih.gov/pubmed/23807574
http://dx.doi.org/10.3390/molecules18077473
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author Cheng, Qiqing
He, Yunfei
Li, Geng
Liu, Yujia
Gao, Wei
Huang, Luqi
author_facet Cheng, Qiqing
He, Yunfei
Li, Geng
Liu, Yujia
Gao, Wei
Huang, Luqi
author_sort Cheng, Qiqing
collection PubMed
description Tanshinones are abietane-type norditerpenoid quinone natural products found in a well-known traditional Chinese medicinal herb, Salvia miltiorrhiza Bunge. The copalyl diphosphate synthase of S. miltiorrhiza (SmCPS) is the key enzyme in the first step for transformation of geranylgeranyl diphosphate (GGPP) into miltiradiene, which has recently been identified as the precursor of tanshinones. Based on previous gene-to-metabolite network, this study examined the influences of various combined elicitors on the expression of SmCPS and production of tanshinones in S. miltiorrhiza hairy root cultures. Combined elicitors were composed of three classes of elicitors, a heavy metal ion (Ag(+)), a polysaccharide (yeast extract, YE), and a plant response-signalling compound (methyl jasmonate, MJ). YE + Ag(+), Ag(+) + MJ, YE + MJ, and YE + Ag(+) + MJ were the combinations we tested. The effect of elicitors on the SmCPS expression level was detected by quantitative real-time PCR (qRT-PCR), and the tanshinones accumulation responses to elicitation were analysed by Ultra Performance Liquid Chromatography (UPLC) metabolite profiling. Of these combined elicitors, the expression of SmCPS was significantly enhanced by elicitation, especially at 24 h and 36 h. Of four tanshinones detected, the contents of cryptotanshinone and dihydrotanshinone I were enhanced by treatment with YE + Ag(+), Ag(+) + MJ, and YE + Ag(+) + MJ. Our results indicate that appropriate combined elicitors can enhance tanshinones production in hairy root cultures.
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spelling pubmed-62697452018-12-17 Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures Cheng, Qiqing He, Yunfei Li, Geng Liu, Yujia Gao, Wei Huang, Luqi Molecules Article Tanshinones are abietane-type norditerpenoid quinone natural products found in a well-known traditional Chinese medicinal herb, Salvia miltiorrhiza Bunge. The copalyl diphosphate synthase of S. miltiorrhiza (SmCPS) is the key enzyme in the first step for transformation of geranylgeranyl diphosphate (GGPP) into miltiradiene, which has recently been identified as the precursor of tanshinones. Based on previous gene-to-metabolite network, this study examined the influences of various combined elicitors on the expression of SmCPS and production of tanshinones in S. miltiorrhiza hairy root cultures. Combined elicitors were composed of three classes of elicitors, a heavy metal ion (Ag(+)), a polysaccharide (yeast extract, YE), and a plant response-signalling compound (methyl jasmonate, MJ). YE + Ag(+), Ag(+) + MJ, YE + MJ, and YE + Ag(+) + MJ were the combinations we tested. The effect of elicitors on the SmCPS expression level was detected by quantitative real-time PCR (qRT-PCR), and the tanshinones accumulation responses to elicitation were analysed by Ultra Performance Liquid Chromatography (UPLC) metabolite profiling. Of these combined elicitors, the expression of SmCPS was significantly enhanced by elicitation, especially at 24 h and 36 h. Of four tanshinones detected, the contents of cryptotanshinone and dihydrotanshinone I were enhanced by treatment with YE + Ag(+), Ag(+) + MJ, and YE + Ag(+) + MJ. Our results indicate that appropriate combined elicitors can enhance tanshinones production in hairy root cultures. MDPI 2013-06-27 /pmc/articles/PMC6269745/ /pubmed/23807574 http://dx.doi.org/10.3390/molecules18077473 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Cheng, Qiqing
He, Yunfei
Li, Geng
Liu, Yujia
Gao, Wei
Huang, Luqi
Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures
title Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures
title_full Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures
title_fullStr Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures
title_full_unstemmed Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures
title_short Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures
title_sort effects of combined elicitors on tanshinone metabolic profiling and smcps expression in salvia miltiorrhiza hairy root cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269745/
https://www.ncbi.nlm.nih.gov/pubmed/23807574
http://dx.doi.org/10.3390/molecules18077473
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