Cargando…

The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds

A major problem in flower bulb cultivation is weed control. Synthetic herbicides are mainly used, although they cause a range of problems, and integrated weed control through application of naturally occurring allelochemicals would be highly desirable. Flower bulb production creates large amounts of...

Descripción completa

Detalles Bibliográficos
Autores principales: Wahyuni, Dinar S. C., van der Kooy, Frank, Klinkhamer, Peter G. L., Verpoorte, Rob, Leiss, Kirsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269756/
https://www.ncbi.nlm.nih.gov/pubmed/23595089
http://dx.doi.org/10.3390/molecules18044510
_version_ 1783376541589176320
author Wahyuni, Dinar S. C.
van der Kooy, Frank
Klinkhamer, Peter G. L.
Verpoorte, Rob
Leiss, Kirsten
author_facet Wahyuni, Dinar S. C.
van der Kooy, Frank
Klinkhamer, Peter G. L.
Verpoorte, Rob
Leiss, Kirsten
author_sort Wahyuni, Dinar S. C.
collection PubMed
description A major problem in flower bulb cultivation is weed control. Synthetic herbicides are mainly used, although they cause a range of problems, and integrated weed control through application of naturally occurring allelochemicals would be highly desirable. Flower bulb production creates large amounts of leftover biomass. Utilizing this source for weed control may provide new applications of the bulb crops. We therefore screened 33 flower bulb extracts for allelochemical activity against weeds. Several methanol and chloroform extracts were observed to inhibit germination and growth of Senecio vulgaris L. and Lolium perenne L., as representatives of di- and mono-cotyledonous weeds, respectively. Narciclasine was identified as the bioactive compound in Narcissus. The extract of Amaryllis belladonna L. was equally active, but did not contain any narciclasine. Bioassay-guided fractionation of the A. belladonna extract resulted in the identification of lycorine as the bio-active compound. The IC(50) measured for radicle growth inhibition was 0.10 µM for narciclasine and 0.93 µM for lycorine, compared to 0.11 mM of chlorpropham, a synthetic herbicide. Therefore, the leftover biomass from the spring bulb industry represents an interesting potential source for promising allelochemicals for further studies on weed growth inhibition.
format Online
Article
Text
id pubmed-6269756
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62697562018-12-14 The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds Wahyuni, Dinar S. C. van der Kooy, Frank Klinkhamer, Peter G. L. Verpoorte, Rob Leiss, Kirsten Molecules Article A major problem in flower bulb cultivation is weed control. Synthetic herbicides are mainly used, although they cause a range of problems, and integrated weed control through application of naturally occurring allelochemicals would be highly desirable. Flower bulb production creates large amounts of leftover biomass. Utilizing this source for weed control may provide new applications of the bulb crops. We therefore screened 33 flower bulb extracts for allelochemical activity against weeds. Several methanol and chloroform extracts were observed to inhibit germination and growth of Senecio vulgaris L. and Lolium perenne L., as representatives of di- and mono-cotyledonous weeds, respectively. Narciclasine was identified as the bioactive compound in Narcissus. The extract of Amaryllis belladonna L. was equally active, but did not contain any narciclasine. Bioassay-guided fractionation of the A. belladonna extract resulted in the identification of lycorine as the bio-active compound. The IC(50) measured for radicle growth inhibition was 0.10 µM for narciclasine and 0.93 µM for lycorine, compared to 0.11 mM of chlorpropham, a synthetic herbicide. Therefore, the leftover biomass from the spring bulb industry represents an interesting potential source for promising allelochemicals for further studies on weed growth inhibition. MDPI 2013-04-17 /pmc/articles/PMC6269756/ /pubmed/23595089 http://dx.doi.org/10.3390/molecules18044510 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wahyuni, Dinar S. C.
van der Kooy, Frank
Klinkhamer, Peter G. L.
Verpoorte, Rob
Leiss, Kirsten
The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds
title The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds
title_full The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds
title_fullStr The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds
title_full_unstemmed The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds
title_short The Use of Bio-Guided Fractionation to Explore the Use of Leftover Biomass in Dutch Flower Bulb Production as Allelochemicals against Weeds
title_sort use of bio-guided fractionation to explore the use of leftover biomass in dutch flower bulb production as allelochemicals against weeds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269756/
https://www.ncbi.nlm.nih.gov/pubmed/23595089
http://dx.doi.org/10.3390/molecules18044510
work_keys_str_mv AT wahyunidinarsc theuseofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds
AT vanderkooyfrank theuseofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds
AT klinkhamerpetergl theuseofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds
AT verpoorterob theuseofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds
AT leisskirsten theuseofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds
AT wahyunidinarsc useofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds
AT vanderkooyfrank useofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds
AT klinkhamerpetergl useofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds
AT verpoorterob useofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds
AT leisskirsten useofbioguidedfractionationtoexploretheuseofleftoverbiomassindutchflowerbulbproductionasallelochemicalsagainstweeds