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Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)
Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269817/ https://www.ncbi.nlm.nih.gov/pubmed/24064448 http://dx.doi.org/10.3390/molecules180910320 |
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author | Passinho-Soares, Helna C. Meira, Paloma R. David, Juceni P. Mesquita, Paulo R. R. do Vale, Ademir E. de M. Rodrigues, Frederico de P. Pereira, Pedro A. de Santana, José Raniere F. de Oliveira, Fabio S. de Andrade, Jailson B. David, Jorge M. |
author_facet | Passinho-Soares, Helna C. Meira, Paloma R. David, Juceni P. Mesquita, Paulo R. R. do Vale, Ademir E. de M. Rodrigues, Frederico de P. Pereira, Pedro A. de Santana, José Raniere F. de Oliveira, Fabio S. de Andrade, Jailson B. David, Jorge M. |
author_sort | Passinho-Soares, Helna C. |
collection | PubMed |
description | Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) on the induction of callus from nodal segments of Plectranthus ornatus Codd and in the production of volatile organic compounds (monoterpenes and sesquiterpenes). The 20 and 40 day calli were subjected to solid phase micro extraction (HS-SPME) and submitted to GCMS analysis. Variations in VOCs between the samples were observed and, a direct relationship was observed between of the major constituent detected (α-terpinyl acetate) and the monoterpenes α-thujene, α-pinene, β-pinene, camphene, sabinene and α-limonene that were present in the volatile fractions. Besides α-terpinyl acetate, isobornyl acetate and α-limonene were also major constituents. Variations were observed in VOCs in the analyzed periods. The best cultivation media for the production of VOCs was found to be MS0 (control). Moderate success was achieved by treatment with 2.68 µM and 5:37 µM NAA (Group 2). With 2,4-D (9.0 µM), only the presence of α-terpinyl acetate and isocumene were detected and, with 2.26 µM of 2,4-D was produced mainly α-terpinyl acetate, α-thujene and β-caryophyllene (16.2%). The VOC profiles present in P. ornatus were interpreted using PCA and HCA. The results permitted us to determine the best cultivation media for VOC production and, the PCA and HCA analysis allowed us to recognize four groups among the different treatments from the compounds identified in this set of treatments. |
format | Online Article Text |
id | pubmed-6269817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62698172018-12-18 Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae) Passinho-Soares, Helna C. Meira, Paloma R. David, Juceni P. Mesquita, Paulo R. R. do Vale, Ademir E. de M. Rodrigues, Frederico de P. Pereira, Pedro A. de Santana, José Raniere F. de Oliveira, Fabio S. de Andrade, Jailson B. David, Jorge M. Molecules Article Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) on the induction of callus from nodal segments of Plectranthus ornatus Codd and in the production of volatile organic compounds (monoterpenes and sesquiterpenes). The 20 and 40 day calli were subjected to solid phase micro extraction (HS-SPME) and submitted to GCMS analysis. Variations in VOCs between the samples were observed and, a direct relationship was observed between of the major constituent detected (α-terpinyl acetate) and the monoterpenes α-thujene, α-pinene, β-pinene, camphene, sabinene and α-limonene that were present in the volatile fractions. Besides α-terpinyl acetate, isobornyl acetate and α-limonene were also major constituents. Variations were observed in VOCs in the analyzed periods. The best cultivation media for the production of VOCs was found to be MS0 (control). Moderate success was achieved by treatment with 2.68 µM and 5:37 µM NAA (Group 2). With 2,4-D (9.0 µM), only the presence of α-terpinyl acetate and isocumene were detected and, with 2.26 µM of 2,4-D was produced mainly α-terpinyl acetate, α-thujene and β-caryophyllene (16.2%). The VOC profiles present in P. ornatus were interpreted using PCA and HCA. The results permitted us to determine the best cultivation media for VOC production and, the PCA and HCA analysis allowed us to recognize four groups among the different treatments from the compounds identified in this set of treatments. MDPI 2013-08-26 /pmc/articles/PMC6269817/ /pubmed/24064448 http://dx.doi.org/10.3390/molecules180910320 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Passinho-Soares, Helna C. Meira, Paloma R. David, Juceni P. Mesquita, Paulo R. R. do Vale, Ademir E. de M. Rodrigues, Frederico de P. Pereira, Pedro A. de Santana, José Raniere F. de Oliveira, Fabio S. de Andrade, Jailson B. David, Jorge M. Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae) |
title | Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae) |
title_full | Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae) |
title_fullStr | Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae) |
title_full_unstemmed | Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae) |
title_short | Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae) |
title_sort | volatile organic compounds obtained by in vitro callus cultivation of plectranthus ornatus codd. (lamiaceae) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269817/ https://www.ncbi.nlm.nih.gov/pubmed/24064448 http://dx.doi.org/10.3390/molecules180910320 |
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