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Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)

Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid...

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Autores principales: Passinho-Soares, Helna C., Meira, Paloma R., David, Juceni P., Mesquita, Paulo R. R., do Vale, Ademir E., de M. Rodrigues, Frederico, de P. Pereira, Pedro A., de Santana, José Raniere F., de Oliveira, Fabio S., de Andrade, Jailson B., David, Jorge M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269817/
https://www.ncbi.nlm.nih.gov/pubmed/24064448
http://dx.doi.org/10.3390/molecules180910320
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author Passinho-Soares, Helna C.
Meira, Paloma R.
David, Juceni P.
Mesquita, Paulo R. R.
do Vale, Ademir E.
de M. Rodrigues, Frederico
de P. Pereira, Pedro A.
de Santana, José Raniere F.
de Oliveira, Fabio S.
de Andrade, Jailson B.
David, Jorge M.
author_facet Passinho-Soares, Helna C.
Meira, Paloma R.
David, Juceni P.
Mesquita, Paulo R. R.
do Vale, Ademir E.
de M. Rodrigues, Frederico
de P. Pereira, Pedro A.
de Santana, José Raniere F.
de Oliveira, Fabio S.
de Andrade, Jailson B.
David, Jorge M.
author_sort Passinho-Soares, Helna C.
collection PubMed
description Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) on the induction of callus from nodal segments of Plectranthus ornatus Codd and in the production of volatile organic compounds (monoterpenes and sesquiterpenes). The 20 and 40 day calli were subjected to solid phase micro extraction (HS-SPME) and submitted to GCMS analysis. Variations in VOCs between the samples were observed and, a direct relationship was observed between of the major constituent detected (α-terpinyl acetate) and the monoterpenes α-thujene, α-pinene, β-pinene, camphene, sabinene and α-limonene that were present in the volatile fractions. Besides α-terpinyl acetate, isobornyl acetate and α-limonene were also major constituents. Variations were observed in VOCs in the analyzed periods. The best cultivation media for the production of VOCs was found to be MS0 (control). Moderate success was achieved by treatment with 2.68 µM and 5:37 µM NAA (Group 2). With 2,4-D (9.0 µM), only the presence of α-terpinyl acetate and isocumene were detected and, with 2.26 µM of 2,4-D was produced mainly α-terpinyl acetate, α-thujene and β-caryophyllene (16.2%). The VOC profiles present in P. ornatus were interpreted using PCA and HCA. The results permitted us to determine the best cultivation media for VOC production and, the PCA and HCA analysis allowed us to recognize four groups among the different treatments from the compounds identified in this set of treatments.
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spelling pubmed-62698172018-12-18 Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae) Passinho-Soares, Helna C. Meira, Paloma R. David, Juceni P. Mesquita, Paulo R. R. do Vale, Ademir E. de M. Rodrigues, Frederico de P. Pereira, Pedro A. de Santana, José Raniere F. de Oliveira, Fabio S. de Andrade, Jailson B. David, Jorge M. Molecules Article Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) on the induction of callus from nodal segments of Plectranthus ornatus Codd and in the production of volatile organic compounds (monoterpenes and sesquiterpenes). The 20 and 40 day calli were subjected to solid phase micro extraction (HS-SPME) and submitted to GCMS analysis. Variations in VOCs between the samples were observed and, a direct relationship was observed between of the major constituent detected (α-terpinyl acetate) and the monoterpenes α-thujene, α-pinene, β-pinene, camphene, sabinene and α-limonene that were present in the volatile fractions. Besides α-terpinyl acetate, isobornyl acetate and α-limonene were also major constituents. Variations were observed in VOCs in the analyzed periods. The best cultivation media for the production of VOCs was found to be MS0 (control). Moderate success was achieved by treatment with 2.68 µM and 5:37 µM NAA (Group 2). With 2,4-D (9.0 µM), only the presence of α-terpinyl acetate and isocumene were detected and, with 2.26 µM of 2,4-D was produced mainly α-terpinyl acetate, α-thujene and β-caryophyllene (16.2%). The VOC profiles present in P. ornatus were interpreted using PCA and HCA. The results permitted us to determine the best cultivation media for VOC production and, the PCA and HCA analysis allowed us to recognize four groups among the different treatments from the compounds identified in this set of treatments. MDPI 2013-08-26 /pmc/articles/PMC6269817/ /pubmed/24064448 http://dx.doi.org/10.3390/molecules180910320 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Passinho-Soares, Helna C.
Meira, Paloma R.
David, Juceni P.
Mesquita, Paulo R. R.
do Vale, Ademir E.
de M. Rodrigues, Frederico
de P. Pereira, Pedro A.
de Santana, José Raniere F.
de Oliveira, Fabio S.
de Andrade, Jailson B.
David, Jorge M.
Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)
title Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)
title_full Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)
title_fullStr Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)
title_full_unstemmed Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)
title_short Volatile Organic Compounds Obtained by in Vitro Callus Cultivation of Plectranthus ornatus Codd. (Lamiaceae)
title_sort volatile organic compounds obtained by in vitro callus cultivation of plectranthus ornatus codd. (lamiaceae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269817/
https://www.ncbi.nlm.nih.gov/pubmed/24064448
http://dx.doi.org/10.3390/molecules180910320
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