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Use of Pyrogallol Red and Pyranine as Probes to Evaluate Antioxidant Capacities towards Hypochlorite

Hypochlorite is a strong oxidant able to induce deleterious effects in biological systems. The goal of this work was to investigate the use of PGR and PYR as probes in assays aimed at evaluating antioxidant activities towards hypochorite and apply it to plant extracts employed in Chilean folk medici...

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Autores principales: Pérez-Cruz, Fernanda, Cortés, Contanza, Atala, Elias, Bohle, Pamela, Valenzuela, Francisco, Olea-Azar, Claudio, Speisky, Hernán, Aspée, Alexis, Lissi, Eduardo, López-Alarcón, Camilo, Bridi, Raquel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269878/
https://www.ncbi.nlm.nih.gov/pubmed/23358322
http://dx.doi.org/10.3390/molecules18021638
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author Pérez-Cruz, Fernanda
Cortés, Contanza
Atala, Elias
Bohle, Pamela
Valenzuela, Francisco
Olea-Azar, Claudio
Speisky, Hernán
Aspée, Alexis
Lissi, Eduardo
López-Alarcón, Camilo
Bridi, Raquel
author_facet Pérez-Cruz, Fernanda
Cortés, Contanza
Atala, Elias
Bohle, Pamela
Valenzuela, Francisco
Olea-Azar, Claudio
Speisky, Hernán
Aspée, Alexis
Lissi, Eduardo
López-Alarcón, Camilo
Bridi, Raquel
author_sort Pérez-Cruz, Fernanda
collection PubMed
description Hypochlorite is a strong oxidant able to induce deleterious effects in biological systems. The goal of this work was to investigate the use of PGR and PYR as probes in assays aimed at evaluating antioxidant activities towards hypochorite and apply it to plant extracts employed in Chilean folk medicine. The consumption of PGR and PYR was evaluated from the decrease in the visible absorbance and fluorescence intensity, respectively. Total phenolic content was determined by the Folin Ciocalteau assay. PGR and PYR react with hypochlorite with different kinetics, being considerably faster the consumption of PGR. Different stoichiometric values were also determined: 0.7 molecules of PGR and 0.33 molecules of PYR were bleached per each molecule of added hypochlorite. Both probes were protected by antioxidants, but the rate of PGR bleaching was too fast to perform a kinetic analysis. For PYR, the protection took place without changes in its initial consumption rate, suggesting a competition between the dye and the antioxidant for hypochlorite. Plant extracts protected PYR giving a PYR-HOCl index that follows the order: Fuchsia magellanica ≈ Marrubium vulgare ≈ Tagetes minuta > Chenopodium ambrosoides ≈ Satureja montana > Thymus praecox. Based on both the kinetic data and the protection afforded by pure antioxidants, we selected PYR as the best probe. The proposed methodology allows evaluating an antioxidant capacity index of plant extracts related to the reactivity of the samples towards hypochlorite.
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spelling pubmed-62698782018-12-14 Use of Pyrogallol Red and Pyranine as Probes to Evaluate Antioxidant Capacities towards Hypochlorite Pérez-Cruz, Fernanda Cortés, Contanza Atala, Elias Bohle, Pamela Valenzuela, Francisco Olea-Azar, Claudio Speisky, Hernán Aspée, Alexis Lissi, Eduardo López-Alarcón, Camilo Bridi, Raquel Molecules Article Hypochlorite is a strong oxidant able to induce deleterious effects in biological systems. The goal of this work was to investigate the use of PGR and PYR as probes in assays aimed at evaluating antioxidant activities towards hypochorite and apply it to plant extracts employed in Chilean folk medicine. The consumption of PGR and PYR was evaluated from the decrease in the visible absorbance and fluorescence intensity, respectively. Total phenolic content was determined by the Folin Ciocalteau assay. PGR and PYR react with hypochlorite with different kinetics, being considerably faster the consumption of PGR. Different stoichiometric values were also determined: 0.7 molecules of PGR and 0.33 molecules of PYR were bleached per each molecule of added hypochlorite. Both probes were protected by antioxidants, but the rate of PGR bleaching was too fast to perform a kinetic analysis. For PYR, the protection took place without changes in its initial consumption rate, suggesting a competition between the dye and the antioxidant for hypochlorite. Plant extracts protected PYR giving a PYR-HOCl index that follows the order: Fuchsia magellanica ≈ Marrubium vulgare ≈ Tagetes minuta > Chenopodium ambrosoides ≈ Satureja montana > Thymus praecox. Based on both the kinetic data and the protection afforded by pure antioxidants, we selected PYR as the best probe. The proposed methodology allows evaluating an antioxidant capacity index of plant extracts related to the reactivity of the samples towards hypochlorite. MDPI 2013-01-28 /pmc/articles/PMC6269878/ /pubmed/23358322 http://dx.doi.org/10.3390/molecules18021638 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Pérez-Cruz, Fernanda
Cortés, Contanza
Atala, Elias
Bohle, Pamela
Valenzuela, Francisco
Olea-Azar, Claudio
Speisky, Hernán
Aspée, Alexis
Lissi, Eduardo
López-Alarcón, Camilo
Bridi, Raquel
Use of Pyrogallol Red and Pyranine as Probes to Evaluate Antioxidant Capacities towards Hypochlorite
title Use of Pyrogallol Red and Pyranine as Probes to Evaluate Antioxidant Capacities towards Hypochlorite
title_full Use of Pyrogallol Red and Pyranine as Probes to Evaluate Antioxidant Capacities towards Hypochlorite
title_fullStr Use of Pyrogallol Red and Pyranine as Probes to Evaluate Antioxidant Capacities towards Hypochlorite
title_full_unstemmed Use of Pyrogallol Red and Pyranine as Probes to Evaluate Antioxidant Capacities towards Hypochlorite
title_short Use of Pyrogallol Red and Pyranine as Probes to Evaluate Antioxidant Capacities towards Hypochlorite
title_sort use of pyrogallol red and pyranine as probes to evaluate antioxidant capacities towards hypochlorite
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269878/
https://www.ncbi.nlm.nih.gov/pubmed/23358322
http://dx.doi.org/10.3390/molecules18021638
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