Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MS(n) and Their Inhibitory Effect on Human Neutrophil Elastase Activity

Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We...

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Detalles Bibliográficos
Autores principales: Huang, Youwu, Chen, Liang, Feng, Li, Guo, Fujiang, Li, Yiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269884/
https://www.ncbi.nlm.nih.gov/pubmed/23807579
http://dx.doi.org/10.3390/molecules18077549
Descripción
Sumario:Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We have now found that S. suberectus contains about 2% phenolic components and characterized the major phenolic components as homogeneous B-type procyanidin conjugates using a liquid chromatography with diode-array detection-ESI mass spectrometry (LC-DAD/ESI-MS) method. This is the first report on occurrence of most B-type procyanidins in this herb. Moreover, the total phenolics extract was assayed for inhibitory activity on human neutrophil elastase and its IC(50) was found to be 1.33 μg/mL.

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