Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MS(n) and Their Inhibitory Effect on Human Neutrophil Elastase Activity

Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We...

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Autores principales: Huang, Youwu, Chen, Liang, Feng, Li, Guo, Fujiang, Li, Yiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269884/
https://www.ncbi.nlm.nih.gov/pubmed/23807579
http://dx.doi.org/10.3390/molecules18077549
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author Huang, Youwu
Chen, Liang
Feng, Li
Guo, Fujiang
Li, Yiming
author_facet Huang, Youwu
Chen, Liang
Feng, Li
Guo, Fujiang
Li, Yiming
author_sort Huang, Youwu
collection PubMed
description Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We have now found that S. suberectus contains about 2% phenolic components and characterized the major phenolic components as homogeneous B-type procyanidin conjugates using a liquid chromatography with diode-array detection-ESI mass spectrometry (LC-DAD/ESI-MS) method. This is the first report on occurrence of most B-type procyanidins in this herb. Moreover, the total phenolics extract was assayed for inhibitory activity on human neutrophil elastase and its IC(50) was found to be 1.33 μg/mL.
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spelling pubmed-62698842018-12-17 Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MS(n) and Their Inhibitory Effect on Human Neutrophil Elastase Activity Huang, Youwu Chen, Liang Feng, Li Guo, Fujiang Li, Yiming Molecules Article Spatholobus suberectus Dunn, belonging to the legume family (Fabaceae), has been used as a Traditional Chinese Medicine for the treatment of anemia, menoxenia and rheumatism. A limited number of studies report that various types of flavonoids are the main characteristic constituents of this herb. We have now found that S. suberectus contains about 2% phenolic components and characterized the major phenolic components as homogeneous B-type procyanidin conjugates using a liquid chromatography with diode-array detection-ESI mass spectrometry (LC-DAD/ESI-MS) method. This is the first report on occurrence of most B-type procyanidins in this herb. Moreover, the total phenolics extract was assayed for inhibitory activity on human neutrophil elastase and its IC(50) was found to be 1.33 μg/mL. MDPI 2013-06-27 /pmc/articles/PMC6269884/ /pubmed/23807579 http://dx.doi.org/10.3390/molecules18077549 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Huang, Youwu
Chen, Liang
Feng, Li
Guo, Fujiang
Li, Yiming
Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MS(n) and Their Inhibitory Effect on Human Neutrophil Elastase Activity
title Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MS(n) and Their Inhibitory Effect on Human Neutrophil Elastase Activity
title_full Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MS(n) and Their Inhibitory Effect on Human Neutrophil Elastase Activity
title_fullStr Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MS(n) and Their Inhibitory Effect on Human Neutrophil Elastase Activity
title_full_unstemmed Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MS(n) and Their Inhibitory Effect on Human Neutrophil Elastase Activity
title_short Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MS(n) and Their Inhibitory Effect on Human Neutrophil Elastase Activity
title_sort characterization of total phenolic constituents from the stems of spatholobus suberectus using lc-dad-ms(n) and their inhibitory effect on human neutrophil elastase activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269884/
https://www.ncbi.nlm.nih.gov/pubmed/23807579
http://dx.doi.org/10.3390/molecules18077549
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