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Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins

Ustiloxins are cyclopeptide mycotoxins produced by Villosiclava virens, the pathogenic fungus of rice false smut disease. Both resins SP207 and SP700 were screened to show the best adsorption and desorption properties for ustiloxins A and B among 20 commercial macroporous resins. Dynamic adsorption...

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Autores principales: Shan, Tijiang, Sun, Weibo, Wang, Xiaohan, Fu, Xiaoxiang, Sun, Wenxian, Zhou, Ligang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269941/
https://www.ncbi.nlm.nih.gov/pubmed/23852165
http://dx.doi.org/10.3390/molecules18078181
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author Shan, Tijiang
Sun, Weibo
Wang, Xiaohan
Fu, Xiaoxiang
Sun, Wenxian
Zhou, Ligang
author_facet Shan, Tijiang
Sun, Weibo
Wang, Xiaohan
Fu, Xiaoxiang
Sun, Wenxian
Zhou, Ligang
author_sort Shan, Tijiang
collection PubMed
description Ustiloxins are cyclopeptide mycotoxins produced by Villosiclava virens, the pathogenic fungus of rice false smut disease. Both resins SP207 and SP700 were screened to show the best adsorption and desorption properties for ustiloxins A and B among 20 commercial macroporous resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions for adsorption of resin SP207 were a processing volume as 32 bed volumes (BV), pH value of 4, and flow rate of 2 BV/h; and those for desorption of resin SP207 were a 40:60 (v/v) ratio of ethanol to water, an eluent volume of 4 BV, pH value of 4 and a flow rate of 3 BV/h. The optimal conditions for adsorption of resin SP700 were a processing volume of 26 BV, pH value as 4, flow rate of 2 BV/h; and those for desorption of resin SP700 were a 30:70 (v/v) ratio of ethanol to water solution as eluent, volume of 4 BV, pH value as 4 and flow rate of 2 BV/h. Under the optimal conditions; the purities of ustiloxins A and B obtained with resin SP207 increased 23.06-fold and 19.78-fold, respectively; and their recoveries were 96.67% and 81.25%; respectively. Similarly; the purities of ustiloxins A and B obtained with resin SP700 increased 14.75-fold and 15.33-fold and their recoveries were 93.65% and 88.64%; respectively. The results show that adsorption and desorption on SP207 and SP700 resins are effective strategies for purifying ustiloxins A and B. The developed methods are beneficial for large-scale preparation and purification of ustiloxins A and B from rice false smut balls.
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spelling pubmed-62699412018-12-17 Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins Shan, Tijiang Sun, Weibo Wang, Xiaohan Fu, Xiaoxiang Sun, Wenxian Zhou, Ligang Molecules Article Ustiloxins are cyclopeptide mycotoxins produced by Villosiclava virens, the pathogenic fungus of rice false smut disease. Both resins SP207 and SP700 were screened to show the best adsorption and desorption properties for ustiloxins A and B among 20 commercial macroporous resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions for adsorption of resin SP207 were a processing volume as 32 bed volumes (BV), pH value of 4, and flow rate of 2 BV/h; and those for desorption of resin SP207 were a 40:60 (v/v) ratio of ethanol to water, an eluent volume of 4 BV, pH value of 4 and a flow rate of 3 BV/h. The optimal conditions for adsorption of resin SP700 were a processing volume of 26 BV, pH value as 4, flow rate of 2 BV/h; and those for desorption of resin SP700 were a 30:70 (v/v) ratio of ethanol to water solution as eluent, volume of 4 BV, pH value as 4 and flow rate of 2 BV/h. Under the optimal conditions; the purities of ustiloxins A and B obtained with resin SP207 increased 23.06-fold and 19.78-fold, respectively; and their recoveries were 96.67% and 81.25%; respectively. Similarly; the purities of ustiloxins A and B obtained with resin SP700 increased 14.75-fold and 15.33-fold and their recoveries were 93.65% and 88.64%; respectively. The results show that adsorption and desorption on SP207 and SP700 resins are effective strategies for purifying ustiloxins A and B. The developed methods are beneficial for large-scale preparation and purification of ustiloxins A and B from rice false smut balls. MDPI 2013-07-11 /pmc/articles/PMC6269941/ /pubmed/23852165 http://dx.doi.org/10.3390/molecules18078181 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Shan, Tijiang
Sun, Weibo
Wang, Xiaohan
Fu, Xiaoxiang
Sun, Wenxian
Zhou, Ligang
Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins
title Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins
title_full Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins
title_fullStr Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins
title_full_unstemmed Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins
title_short Purification of Ustiloxins A and B from Rice False Smut Balls by Macroporous Resins
title_sort purification of ustiloxins a and b from rice false smut balls by macroporous resins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6269941/
https://www.ncbi.nlm.nih.gov/pubmed/23852165
http://dx.doi.org/10.3390/molecules18078181
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