Synthesis and Binding Ability of Molecular Probes Based on a Phenanthroline Derivative: Theory and Experiment

A fluorescent and colorimetric molecular probe containing phenol groups has been designed and synthesized. The anion binding ability was evaluated for biolgically important anions (F(−), Cl(−), Br(−), I(−), AcO(−) and H(2)PO(4)(−)) by theoretical investigation, UV-Vis and fluorescence spectroscopy and (1)H-NMR titration experiments. Results indicated the probe showed strong binding ability for H(2)PO(4)(−) without the interference of other anions tested and the interaction process was accompanied by color changes. Theoretical investigation analysis revealed that intramolecular hydrogen bonds existed in the structure of the probe and the roles of molecular frontier orbitals in molecular interplay were determined.