Enzymatic Synthesis of Highly Fluorescent 8-Azapurine Ribosides Using a Purine Nucleoside Phosphorylase Reverse Reaction: Variable Ribosylation Sites

Various forms of purine-nucleoside phosphorylase (PNP) were used as catalysts of enzymatic ribosylation of selected fluorescent 8-azapurines. It was found that the recombinant calf PNP catalyzes ribosylation of 2,6-diamino-8-azapurine in a phosphate-free medium, with ribose-1-phosphate as ribose don...

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Detalles Bibliográficos
Autores principales: Stachelska-Wierzchowska, Alicja, Wierzchowski, Jacek, Wielgus-Kutrowska, Beata, Mikleušević, Goran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270051/
https://www.ncbi.nlm.nih.gov/pubmed/24126376
http://dx.doi.org/10.3390/molecules181012587

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270051/
https://www.ncbi.nlm.nih.gov/pubmed/24126376
http://dx.doi.org/10.3390/molecules181012587

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