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Enzymatic Synthesis of Highly Fluorescent 8-Azapurine Ribosides Using a Purine Nucleoside Phosphorylase Reverse Reaction: Variable Ribosylation Sites
Various forms of purine-nucleoside phosphorylase (PNP) were used as catalysts of enzymatic ribosylation of selected fluorescent 8-azapurines. It was found that the recombinant calf PNP catalyzes ribosylation of 2,6-diamino-8-azapurine in a phosphate-free medium, with ribose-1-phosphate as ribose don...
Autores principales: | Stachelska-Wierzchowska, Alicja, Wierzchowski, Jacek, Wielgus-Kutrowska, Beata, Mikleušević, Goran |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270051/ https://www.ncbi.nlm.nih.gov/pubmed/24126376 http://dx.doi.org/10.3390/molecules181012587 |
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