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Peripheral Antinociception of a Chalcone, Flavokawin B and Possible Involvement of the Nitric Oxide/Cyclic Guanosine Monophosphate/Potassium Channels Pathway

Previous studies have shown that systemic administration of 6'-hydroxy-2',4'-dimethoxychalcone (flavokawin B, FKB) exerts significant peripheral and central antinociceptive effects in laboratory animals. However, the mechanisms underlying these peripheral and central antinociceptive e...

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Autores principales: Kamaldin, Mohd Nasier, Akhtar, Muhammad Nadeem, Mohamad, Azam Shah, Lajis, Nordin, Perimal, Enoch Kumar, Akira, Ahmad, Ming-Tatt, Lee, Israf, Daud Ahmad, Sulaiman, Mohd Roslan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270115/
https://www.ncbi.nlm.nih.gov/pubmed/23612473
http://dx.doi.org/10.3390/molecules18044209
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author Kamaldin, Mohd Nasier
Akhtar, Muhammad Nadeem
Mohamad, Azam Shah
Lajis, Nordin
Perimal, Enoch Kumar
Akira, Ahmad
Ming-Tatt, Lee
Israf, Daud Ahmad
Sulaiman, Mohd Roslan
author_facet Kamaldin, Mohd Nasier
Akhtar, Muhammad Nadeem
Mohamad, Azam Shah
Lajis, Nordin
Perimal, Enoch Kumar
Akira, Ahmad
Ming-Tatt, Lee
Israf, Daud Ahmad
Sulaiman, Mohd Roslan
author_sort Kamaldin, Mohd Nasier
collection PubMed
description Previous studies have shown that systemic administration of 6'-hydroxy-2',4'-dimethoxychalcone (flavokawin B, FKB) exerts significant peripheral and central antinociceptive effects in laboratory animals. However, the mechanisms underlying these peripheral and central antinociceptive effects have yet to be elucidated. Therefore, the objective of the present study was to evaluate the participation of nitric oxide (NO)/cyclic guanosine monophosphate (cGMP)/potassium (K(+)) channels pathway in the peripheral antinociception induced by FKB. It was demonstrated that intraplantar (i.pl.) administration of FKB (150, 250, 375 and 500 µg/paw) resulted in dose-dependent peripheral antinociception against mechanical hyperalgesia in carrageenan-induced hyperalgesia test model in rats. The possibility of FKB having either a central or a systemic effect was excluded since administration of FKB into the right paw did not elicit antinociception in the contralateral paw. Furthermore, peripheral antinociception induced by FKB (500 µg/paw) was significantly reduced when l-arginine (25 µg/paw, i.pl.), Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 50 µg/paw, i.pl.), glibenclamide (300 µg/paw, i.pl.), tetraethylammonium (300 µg/paw, i.pl.) and charybdotoxin (3 µg/paw, i.pl.) were injected before treatment. Taken together, our present data suggest that FKB elicits peripheral antinociception when assessed in the mechanical hyperalgesia induced by carrageenan. In addition, it was also demonstrated that this effect was mediated through interaction of the NO/cGMP/K(+) channels signaling pathway.
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spelling pubmed-62701152018-12-14 Peripheral Antinociception of a Chalcone, Flavokawin B and Possible Involvement of the Nitric Oxide/Cyclic Guanosine Monophosphate/Potassium Channels Pathway Kamaldin, Mohd Nasier Akhtar, Muhammad Nadeem Mohamad, Azam Shah Lajis, Nordin Perimal, Enoch Kumar Akira, Ahmad Ming-Tatt, Lee Israf, Daud Ahmad Sulaiman, Mohd Roslan Molecules Article Previous studies have shown that systemic administration of 6'-hydroxy-2',4'-dimethoxychalcone (flavokawin B, FKB) exerts significant peripheral and central antinociceptive effects in laboratory animals. However, the mechanisms underlying these peripheral and central antinociceptive effects have yet to be elucidated. Therefore, the objective of the present study was to evaluate the participation of nitric oxide (NO)/cyclic guanosine monophosphate (cGMP)/potassium (K(+)) channels pathway in the peripheral antinociception induced by FKB. It was demonstrated that intraplantar (i.pl.) administration of FKB (150, 250, 375 and 500 µg/paw) resulted in dose-dependent peripheral antinociception against mechanical hyperalgesia in carrageenan-induced hyperalgesia test model in rats. The possibility of FKB having either a central or a systemic effect was excluded since administration of FKB into the right paw did not elicit antinociception in the contralateral paw. Furthermore, peripheral antinociception induced by FKB (500 µg/paw) was significantly reduced when l-arginine (25 µg/paw, i.pl.), Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 50 µg/paw, i.pl.), glibenclamide (300 µg/paw, i.pl.), tetraethylammonium (300 µg/paw, i.pl.) and charybdotoxin (3 µg/paw, i.pl.) were injected before treatment. Taken together, our present data suggest that FKB elicits peripheral antinociception when assessed in the mechanical hyperalgesia induced by carrageenan. In addition, it was also demonstrated that this effect was mediated through interaction of the NO/cGMP/K(+) channels signaling pathway. MDPI 2013-04-10 /pmc/articles/PMC6270115/ /pubmed/23612473 http://dx.doi.org/10.3390/molecules18044209 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Kamaldin, Mohd Nasier
Akhtar, Muhammad Nadeem
Mohamad, Azam Shah
Lajis, Nordin
Perimal, Enoch Kumar
Akira, Ahmad
Ming-Tatt, Lee
Israf, Daud Ahmad
Sulaiman, Mohd Roslan
Peripheral Antinociception of a Chalcone, Flavokawin B and Possible Involvement of the Nitric Oxide/Cyclic Guanosine Monophosphate/Potassium Channels Pathway
title Peripheral Antinociception of a Chalcone, Flavokawin B and Possible Involvement of the Nitric Oxide/Cyclic Guanosine Monophosphate/Potassium Channels Pathway
title_full Peripheral Antinociception of a Chalcone, Flavokawin B and Possible Involvement of the Nitric Oxide/Cyclic Guanosine Monophosphate/Potassium Channels Pathway
title_fullStr Peripheral Antinociception of a Chalcone, Flavokawin B and Possible Involvement of the Nitric Oxide/Cyclic Guanosine Monophosphate/Potassium Channels Pathway
title_full_unstemmed Peripheral Antinociception of a Chalcone, Flavokawin B and Possible Involvement of the Nitric Oxide/Cyclic Guanosine Monophosphate/Potassium Channels Pathway
title_short Peripheral Antinociception of a Chalcone, Flavokawin B and Possible Involvement of the Nitric Oxide/Cyclic Guanosine Monophosphate/Potassium Channels Pathway
title_sort peripheral antinociception of a chalcone, flavokawin b and possible involvement of the nitric oxide/cyclic guanosine monophosphate/potassium channels pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270115/
https://www.ncbi.nlm.nih.gov/pubmed/23612473
http://dx.doi.org/10.3390/molecules18044209
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