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Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery

Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes...

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Detalles Bibliográficos
Autores principales: Molnár, Eszter, Kuntam, Soujanya, Cingaram, Pradeep Kumar Reddy, Peksel, Begüm, Suresh, Bhavyashree, Fábián, Gabriella, Fehér, Liliána Z., Bokros, Attila, Medgyesi, Ágnes, Ayaydin, Ferhan, Puskás, László G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270374/
https://www.ncbi.nlm.nih.gov/pubmed/23966084
http://dx.doi.org/10.3390/molecules18089999
Descripción
Sumario:Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1–10 micromolar range, they were non-toxic even during long-term incubations.