Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery

Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes...

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Autores principales: Molnár, Eszter, Kuntam, Soujanya, Cingaram, Pradeep Kumar Reddy, Peksel, Begüm, Suresh, Bhavyashree, Fábián, Gabriella, Fehér, Liliána Z., Bokros, Attila, Medgyesi, Ágnes, Ayaydin, Ferhan, Puskás, László G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270374/
https://www.ncbi.nlm.nih.gov/pubmed/23966084
http://dx.doi.org/10.3390/molecules18089999
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author Molnár, Eszter
Kuntam, Soujanya
Cingaram, Pradeep Kumar Reddy
Peksel, Begüm
Suresh, Bhavyashree
Fábián, Gabriella
Fehér, Liliána Z.
Bokros, Attila
Medgyesi, Ágnes
Ayaydin, Ferhan
Puskás, László G.
author_facet Molnár, Eszter
Kuntam, Soujanya
Cingaram, Pradeep Kumar Reddy
Peksel, Begüm
Suresh, Bhavyashree
Fábián, Gabriella
Fehér, Liliána Z.
Bokros, Attila
Medgyesi, Ágnes
Ayaydin, Ferhan
Puskás, László G.
author_sort Molnár, Eszter
collection PubMed
description Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1–10 micromolar range, they were non-toxic even during long-term incubations.
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spelling pubmed-62703742018-12-18 Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery Molnár, Eszter Kuntam, Soujanya Cingaram, Pradeep Kumar Reddy Peksel, Begüm Suresh, Bhavyashree Fábián, Gabriella Fehér, Liliána Z. Bokros, Attila Medgyesi, Ágnes Ayaydin, Ferhan Puskás, László G. Molecules Article Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to discover novel cell staining fluorescent dyes. Compounds with high native fluorescence were selected from a 14,585-member library and further tested on living cells under the microscope. Eleven compartment-specific, cell-permeable (or plasma membrane-targeted) fluorochromes were identified. Their cytotoxicity was tested and found that between 1–10 micromolar range, they were non-toxic even during long-term incubations. MDPI 2013-08-20 /pmc/articles/PMC6270374/ /pubmed/23966084 http://dx.doi.org/10.3390/molecules18089999 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Molnár, Eszter
Kuntam, Soujanya
Cingaram, Pradeep Kumar Reddy
Peksel, Begüm
Suresh, Bhavyashree
Fábián, Gabriella
Fehér, Liliána Z.
Bokros, Attila
Medgyesi, Ágnes
Ayaydin, Ferhan
Puskás, László G.
Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery
title Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery
title_full Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery
title_fullStr Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery
title_full_unstemmed Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery
title_short Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery
title_sort combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270374/
https://www.ncbi.nlm.nih.gov/pubmed/23966084
http://dx.doi.org/10.3390/molecules18089999
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