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Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp

The solution properties of four fractions (LPI–IV) from crude longan pulp polysaccharides (LP3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with Congo red, and atomic force microscopy. Their radii of gyration (<S(2)>(z)(1/2...

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Autores principales: Yi, Yang, Huang, Fei, Zhang, Ming-Wei, Zhang, Rui-Fen, Deng, Yuan-Yuan, Wei, Zhen-Cheng, He, Jing-Ren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270385/
https://www.ncbi.nlm.nih.gov/pubmed/24051475
http://dx.doi.org/10.3390/molecules180911601
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author Yi, Yang
Huang, Fei
Zhang, Ming-Wei
Zhang, Rui-Fen
Deng, Yuan-Yuan
Wei, Zhen-Cheng
He, Jing-Ren
author_facet Yi, Yang
Huang, Fei
Zhang, Ming-Wei
Zhang, Rui-Fen
Deng, Yuan-Yuan
Wei, Zhen-Cheng
He, Jing-Ren
author_sort Yi, Yang
collection PubMed
description The solution properties of four fractions (LPI–IV) from crude longan pulp polysaccharides (LP3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with Congo red, and atomic force microscopy. Their radii of gyration (<S(2)>(z)(1/2)) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of <S(2)>(z)(1/2) = k M(w)(v) were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([η]) were 9.945, 25.38, 308.2 and 452.1 mL/g, respectively. Moreover, the dependence of [η] on M(w) was established to be [η] = 5.3 × 10(−2)M(w)(0.61) (mL/g). LPI had both a sphere-like conformation and a triple-helix structure, and LPII–IV existed as flexible chains. LP3, LPI, LPII and LPIII all exhibited direct inhibitory effects on A549, HeLa and HepG2 cells in a positive dose-dependent manner in the range of 50–400 µg/mL. The activities of LPIII, especially the inhibition of HepG2 cell proliferation, were stronger than those of others, which may be partly related to its flexible conformation. The present results support the cancer therapeutic potential of longan polysaccharides.
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spelling pubmed-62703852018-12-18 Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp Yi, Yang Huang, Fei Zhang, Ming-Wei Zhang, Rui-Fen Deng, Yuan-Yuan Wei, Zhen-Cheng He, Jing-Ren Molecules Article The solution properties of four fractions (LPI–IV) from crude longan pulp polysaccharides (LP3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with Congo red, and atomic force microscopy. Their radii of gyration (<S(2)>(z)(1/2)) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of <S(2)>(z)(1/2) = k M(w)(v) were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([η]) were 9.945, 25.38, 308.2 and 452.1 mL/g, respectively. Moreover, the dependence of [η] on M(w) was established to be [η] = 5.3 × 10(−2)M(w)(0.61) (mL/g). LPI had both a sphere-like conformation and a triple-helix structure, and LPII–IV existed as flexible chains. LP3, LPI, LPII and LPIII all exhibited direct inhibitory effects on A549, HeLa and HepG2 cells in a positive dose-dependent manner in the range of 50–400 µg/mL. The activities of LPIII, especially the inhibition of HepG2 cell proliferation, were stronger than those of others, which may be partly related to its flexible conformation. The present results support the cancer therapeutic potential of longan polysaccharides. MDPI 2013-09-18 /pmc/articles/PMC6270385/ /pubmed/24051475 http://dx.doi.org/10.3390/molecules180911601 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Yi, Yang
Huang, Fei
Zhang, Ming-Wei
Zhang, Rui-Fen
Deng, Yuan-Yuan
Wei, Zhen-Cheng
He, Jing-Ren
Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp
title Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp
title_full Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp
title_fullStr Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp
title_full_unstemmed Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp
title_short Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp
title_sort solution properties and in vitro anti-tumor activities of polysaccharides from longan pulp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270385/
https://www.ncbi.nlm.nih.gov/pubmed/24051475
http://dx.doi.org/10.3390/molecules180911601
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