Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture

This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), we...

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Autores principales: de Souza, Gezimar D., Mithöfer, Axel, Daolio, Cristina, Schneider, Bernd, Rodrigues-Filho, Edson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270395/
https://www.ncbi.nlm.nih.gov/pubmed/23442929
http://dx.doi.org/10.3390/molecules18032528
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author de Souza, Gezimar D.
Mithöfer, Axel
Daolio, Cristina
Schneider, Bernd
Rodrigues-Filho, Edson
author_facet de Souza, Gezimar D.
Mithöfer, Axel
Daolio, Cristina
Schneider, Bernd
Rodrigues-Filho, Edson
author_sort de Souza, Gezimar D.
collection PubMed
description This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC(50) values which range from 0.11 (±0.02) to 4.69 (±0.47) μM showed the high cytotoxicity of these compounds.
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spelling pubmed-62703952018-12-20 Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture de Souza, Gezimar D. Mithöfer, Axel Daolio, Cristina Schneider, Bernd Rodrigues-Filho, Edson Molecules Article This present work describes the application of liquid chromatograpy-solid phase extraction-nuclear magnetic resonance spectroscopy to analyse Alternaria alternata crude extracts. Altenusin (1), alternariol (2), 3'-hydroxyalternariol monomethyl ether (3), and alternariol monomethyl ether (4), were separated and identified. High-resolution mass spectrometry confirmed the proposed structures. The cytotoxic effects of these compounds towards plants were determined using soybean (Glycine max) cell cultures as a model. EC(50) values which range from 0.11 (±0.02) to 4.69 (±0.47) μM showed the high cytotoxicity of these compounds. MDPI 2013-02-26 /pmc/articles/PMC6270395/ /pubmed/23442929 http://dx.doi.org/10.3390/molecules18032528 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
de Souza, Gezimar D.
Mithöfer, Axel
Daolio, Cristina
Schneider, Bernd
Rodrigues-Filho, Edson
Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture
title Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture
title_full Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture
title_fullStr Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture
title_full_unstemmed Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture
title_short Identification of Alternaria alternata Mycotoxins by LC-SPE-NMR and Their Cytotoxic Effects to Soybean (Glycine max) Cell Suspension Culture
title_sort identification of alternaria alternata mycotoxins by lc-spe-nmr and their cytotoxic effects to soybean (glycine max) cell suspension culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270395/
https://www.ncbi.nlm.nih.gov/pubmed/23442929
http://dx.doi.org/10.3390/molecules18032528
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