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Enantioselective Esterification of Ibuprofen under Microwave Irradiation
Enantioselective esterification of ibuprofen has been successfully carried out in an organic solvent catalyzed by recombinant APE 1547 (a thermophilic esterase from the archaeon Aeropyrum pernix K1). Here we used microwave irradiation (MW) as the mode of heating to improve the enzyme performance. Un...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270588/ https://www.ncbi.nlm.nih.gov/pubmed/23669631 http://dx.doi.org/10.3390/molecules18055472 |
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author | Yang, Zecheng Niu, Xuedun Fang, Xuedong Chen, Ge Zhang, Hong Yue, Hong Wang, Lei Zhao, Dantong Wang, Zhi |
author_facet | Yang, Zecheng Niu, Xuedun Fang, Xuedong Chen, Ge Zhang, Hong Yue, Hong Wang, Lei Zhao, Dantong Wang, Zhi |
author_sort | Yang, Zecheng |
collection | PubMed |
description | Enantioselective esterification of ibuprofen has been successfully carried out in an organic solvent catalyzed by recombinant APE 1547 (a thermophilic esterase from the archaeon Aeropyrum pernix K1). Here we used microwave irradiation (MW) as the mode of heating to improve the enzyme performance. Under the optimum conditions, the enzyme activity of APE 1547 was 4.16 μmol/mg/h and the enantioselectivity (E value) was 52.9. Compared with conventional heating, the enzyme activity and the enantioselectivity were increased about 21.9-fold and 1.4-fold, respectively. The results also indicated that APE 1547 can maintain 95% of its activity even after being used five times, suggesting that the enzyme is stable under low power MW conditions. |
format | Online Article Text |
id | pubmed-6270588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62705882018-12-14 Enantioselective Esterification of Ibuprofen under Microwave Irradiation Yang, Zecheng Niu, Xuedun Fang, Xuedong Chen, Ge Zhang, Hong Yue, Hong Wang, Lei Zhao, Dantong Wang, Zhi Molecules Article Enantioselective esterification of ibuprofen has been successfully carried out in an organic solvent catalyzed by recombinant APE 1547 (a thermophilic esterase from the archaeon Aeropyrum pernix K1). Here we used microwave irradiation (MW) as the mode of heating to improve the enzyme performance. Under the optimum conditions, the enzyme activity of APE 1547 was 4.16 μmol/mg/h and the enantioselectivity (E value) was 52.9. Compared with conventional heating, the enzyme activity and the enantioselectivity were increased about 21.9-fold and 1.4-fold, respectively. The results also indicated that APE 1547 can maintain 95% of its activity even after being used five times, suggesting that the enzyme is stable under low power MW conditions. MDPI 2013-05-13 /pmc/articles/PMC6270588/ /pubmed/23669631 http://dx.doi.org/10.3390/molecules18055472 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Yang, Zecheng Niu, Xuedun Fang, Xuedong Chen, Ge Zhang, Hong Yue, Hong Wang, Lei Zhao, Dantong Wang, Zhi Enantioselective Esterification of Ibuprofen under Microwave Irradiation |
title | Enantioselective Esterification of Ibuprofen under Microwave Irradiation |
title_full | Enantioselective Esterification of Ibuprofen under Microwave Irradiation |
title_fullStr | Enantioselective Esterification of Ibuprofen under Microwave Irradiation |
title_full_unstemmed | Enantioselective Esterification of Ibuprofen under Microwave Irradiation |
title_short | Enantioselective Esterification of Ibuprofen under Microwave Irradiation |
title_sort | enantioselective esterification of ibuprofen under microwave irradiation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270588/ https://www.ncbi.nlm.nih.gov/pubmed/23669631 http://dx.doi.org/10.3390/molecules18055472 |
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