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Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi

In order to determine the in vivo activity against the protozoan Trypanosoma cruzi, two doses (50 and 75 mg/kg) of a chloroform extract of Carica papaya seeds were evaluated compared with a control group of allopurinol. The activity of a mixture of the three main compounds (oleic, palmitic and stear...

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Autores principales: Jiménez-Coello, Matilde, Guzman-Marín, Eugenia, Ortega-Pacheco, Antonio, Perez-Gutiérrez, Salud, Acosta-Viana, Karla Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270640/
https://www.ncbi.nlm.nih.gov/pubmed/24126379
http://dx.doi.org/10.3390/molecules181012621
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author Jiménez-Coello, Matilde
Guzman-Marín, Eugenia
Ortega-Pacheco, Antonio
Perez-Gutiérrez, Salud
Acosta-Viana, Karla Y.
author_facet Jiménez-Coello, Matilde
Guzman-Marín, Eugenia
Ortega-Pacheco, Antonio
Perez-Gutiérrez, Salud
Acosta-Viana, Karla Y.
author_sort Jiménez-Coello, Matilde
collection PubMed
description In order to determine the in vivo activity against the protozoan Trypanosoma cruzi, two doses (50 and 75 mg/kg) of a chloroform extract of Carica papaya seeds were evaluated compared with a control group of allopurinol. The activity of a mixture of the three main compounds (oleic, palmitic and stearic acids in a proportion of 45.9% of oleic acid, 24.1% of palmitic and 8.52% of stearic acid previously identified in the crude extract of C. papaya was evaluated at doses of 100, 200 and 300 mg/kg. Both doses of the extracts were orally administered for 28 days. A significant reduction (p < 0.05) in the number of blood trypomastigotes was observed in animals treated with the evaluated doses of the C. papaya extract in comparison with the positive control group (allopurinol 8.5 mg/kg). Parasitemia in animals treated with the fatty acids mixture was also significantly reduced (p < 0.05), compared to negative control animals. These results demonstrate that the fatty acids identified in the seed extracts of C. papaya (from ripe fruit) are able to reduce the number of parasites from both parasite stages, blood trypomastigote and amastigote (intracellular stage).
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spelling pubmed-62706402018-12-18 Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi Jiménez-Coello, Matilde Guzman-Marín, Eugenia Ortega-Pacheco, Antonio Perez-Gutiérrez, Salud Acosta-Viana, Karla Y. Molecules Article In order to determine the in vivo activity against the protozoan Trypanosoma cruzi, two doses (50 and 75 mg/kg) of a chloroform extract of Carica papaya seeds were evaluated compared with a control group of allopurinol. The activity of a mixture of the three main compounds (oleic, palmitic and stearic acids in a proportion of 45.9% of oleic acid, 24.1% of palmitic and 8.52% of stearic acid previously identified in the crude extract of C. papaya was evaluated at doses of 100, 200 and 300 mg/kg. Both doses of the extracts were orally administered for 28 days. A significant reduction (p < 0.05) in the number of blood trypomastigotes was observed in animals treated with the evaluated doses of the C. papaya extract in comparison with the positive control group (allopurinol 8.5 mg/kg). Parasitemia in animals treated with the fatty acids mixture was also significantly reduced (p < 0.05), compared to negative control animals. These results demonstrate that the fatty acids identified in the seed extracts of C. papaya (from ripe fruit) are able to reduce the number of parasites from both parasite stages, blood trypomastigote and amastigote (intracellular stage). MDPI 2013-10-11 /pmc/articles/PMC6270640/ /pubmed/24126379 http://dx.doi.org/10.3390/molecules181012621 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Jiménez-Coello, Matilde
Guzman-Marín, Eugenia
Ortega-Pacheco, Antonio
Perez-Gutiérrez, Salud
Acosta-Viana, Karla Y.
Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi
title Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi
title_full Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi
title_fullStr Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi
title_full_unstemmed Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi
title_short Assessment of the Anti-Protozoal Activity of Crude Carica papaya Seed Extract against Trypanosoma cruzi
title_sort assessment of the anti-protozoal activity of crude carica papaya seed extract against trypanosoma cruzi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270640/
https://www.ncbi.nlm.nih.gov/pubmed/24126379
http://dx.doi.org/10.3390/molecules181012621
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