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Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins

Condensed tannins (CT) from purple prairie clover (PPC; Dalea purpurea Vent.) and sainfoin (SF; Onobrychis viciifolia) were assessed for anti-Escherichia coli activity by comparing their ability to react with proteins and liposome, cause cell aggregation, and alter outer membrane (OM) morphology and...

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Autores principales: Liu, Xiu-Li, Hao, Yong-Qing, Jin, Long, Xu, Zhong-Jun, McAllister, Tim A., Wang, Yuxi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270645/
https://www.ncbi.nlm.nih.gov/pubmed/23396143
http://dx.doi.org/10.3390/molecules18022183
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author Liu, Xiu-Li
Hao, Yong-Qing
Jin, Long
Xu, Zhong-Jun
McAllister, Tim A.
Wang, Yuxi
author_facet Liu, Xiu-Li
Hao, Yong-Qing
Jin, Long
Xu, Zhong-Jun
McAllister, Tim A.
Wang, Yuxi
author_sort Liu, Xiu-Li
collection PubMed
description Condensed tannins (CT) from purple prairie clover (PPC; Dalea purpurea Vent.) and sainfoin (SF; Onobrychis viciifolia) were assessed for anti-Escherichia coli activity by comparing their ability to react with proteins and liposome, cause cell aggregation, and alter outer membrane (OM) morphology and permeability. The PPC CT had greater (P < 0.01) protein-precipitating capacity than SF CT using either bovine serum albumin or ribulose 1,5-disphosphate carboxylase as model proteins. Minimum inhibitory concentration of PPC CT for two strains of E. coli and five strains of E. coli O157:H7 was four to six times lower than that of SF CT. E. coli exposed to 10 µg/mL of both CT had higher (P < 0.05) OM permeability than controls and was greater (P < 0.05) for PPC than for SF CT. Addition of both CT at 50 and 200 µg/mL caused cell aggregation which was more evident (P < 0.05) for PPC than for SF CT. Transmission electron microscopy showed electron dense material on the cell surface when cells were exposed to 50 µg/mL of PPC CT. The greater anti-E. coli activity of PPC than SF CT was due to its enhanced ability to precipitate protein that increased OM permeability and promoted cell aggregation.
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spelling pubmed-62706452018-12-14 Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins Liu, Xiu-Li Hao, Yong-Qing Jin, Long Xu, Zhong-Jun McAllister, Tim A. Wang, Yuxi Molecules Article Condensed tannins (CT) from purple prairie clover (PPC; Dalea purpurea Vent.) and sainfoin (SF; Onobrychis viciifolia) were assessed for anti-Escherichia coli activity by comparing their ability to react with proteins and liposome, cause cell aggregation, and alter outer membrane (OM) morphology and permeability. The PPC CT had greater (P < 0.01) protein-precipitating capacity than SF CT using either bovine serum albumin or ribulose 1,5-disphosphate carboxylase as model proteins. Minimum inhibitory concentration of PPC CT for two strains of E. coli and five strains of E. coli O157:H7 was four to six times lower than that of SF CT. E. coli exposed to 10 µg/mL of both CT had higher (P < 0.05) OM permeability than controls and was greater (P < 0.05) for PPC than for SF CT. Addition of both CT at 50 and 200 µg/mL caused cell aggregation which was more evident (P < 0.05) for PPC than for SF CT. Transmission electron microscopy showed electron dense material on the cell surface when cells were exposed to 50 µg/mL of PPC CT. The greater anti-E. coli activity of PPC than SF CT was due to its enhanced ability to precipitate protein that increased OM permeability and promoted cell aggregation. MDPI 2013-02-08 /pmc/articles/PMC6270645/ /pubmed/23396143 http://dx.doi.org/10.3390/molecules18022183 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Liu, Xiu-Li
Hao, Yong-Qing
Jin, Long
Xu, Zhong-Jun
McAllister, Tim A.
Wang, Yuxi
Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins
title Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins
title_full Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins
title_fullStr Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins
title_full_unstemmed Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins
title_short Anti-Escherichia coli O157:H7 Properties of Purple Prairie Clover and Sainfoin Condensed Tannins
title_sort anti-escherichia coli o157:h7 properties of purple prairie clover and sainfoin condensed tannins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270645/
https://www.ncbi.nlm.nih.gov/pubmed/23396143
http://dx.doi.org/10.3390/molecules18022183
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