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Production of Anti-Cancer Agent Using Microbial Biotransformation
Microbial biotransformation is a great model system to produce drugs and biologically active compounds. In this study, we elucidated the fermentation and production of an anti-cancer agent from a microbial process for regiospecific hydroxylation of resveratrol. Among the strains examined, a potent s...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270657/ https://www.ncbi.nlm.nih.gov/pubmed/25325153 http://dx.doi.org/10.3390/molecules191016684 |
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author | Roh, Changhyun Kang, ChanKyu |
author_facet | Roh, Changhyun Kang, ChanKyu |
author_sort | Roh, Changhyun |
collection | PubMed |
description | Microbial biotransformation is a great model system to produce drugs and biologically active compounds. In this study, we elucidated the fermentation and production of an anti-cancer agent from a microbial process for regiospecific hydroxylation of resveratrol. Among the strains examined, a potent strain showed high regiospecific hydroxylation activity to produce piceatannol. In a 5 L (w/v 3 L) jar fermentation, this wild type Streptomyces sp. in the batch system produced 205 mg of piceatannol (i.e., 60% yields) from 342 mg of resveratrol in 20 h. Using the product, an in vitro anti-cancer study was performed against a human cancer cell line (HeLa). It showed that the biotransformed piceatannol possessed a significant anticancer activity. This result demonstrates that a biotransformation screening method might be of therapeutic interest with respect to the identification of anti-cancer drugs. |
format | Online Article Text |
id | pubmed-6270657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62706572018-12-27 Production of Anti-Cancer Agent Using Microbial Biotransformation Roh, Changhyun Kang, ChanKyu Molecules Communication Microbial biotransformation is a great model system to produce drugs and biologically active compounds. In this study, we elucidated the fermentation and production of an anti-cancer agent from a microbial process for regiospecific hydroxylation of resveratrol. Among the strains examined, a potent strain showed high regiospecific hydroxylation activity to produce piceatannol. In a 5 L (w/v 3 L) jar fermentation, this wild type Streptomyces sp. in the batch system produced 205 mg of piceatannol (i.e., 60% yields) from 342 mg of resveratrol in 20 h. Using the product, an in vitro anti-cancer study was performed against a human cancer cell line (HeLa). It showed that the biotransformed piceatannol possessed a significant anticancer activity. This result demonstrates that a biotransformation screening method might be of therapeutic interest with respect to the identification of anti-cancer drugs. MDPI 2014-10-16 /pmc/articles/PMC6270657/ /pubmed/25325153 http://dx.doi.org/10.3390/molecules191016684 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Roh, Changhyun Kang, ChanKyu Production of Anti-Cancer Agent Using Microbial Biotransformation |
title | Production of Anti-Cancer Agent Using Microbial Biotransformation |
title_full | Production of Anti-Cancer Agent Using Microbial Biotransformation |
title_fullStr | Production of Anti-Cancer Agent Using Microbial Biotransformation |
title_full_unstemmed | Production of Anti-Cancer Agent Using Microbial Biotransformation |
title_short | Production of Anti-Cancer Agent Using Microbial Biotransformation |
title_sort | production of anti-cancer agent using microbial biotransformation |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270657/ https://www.ncbi.nlm.nih.gov/pubmed/25325153 http://dx.doi.org/10.3390/molecules191016684 |
work_keys_str_mv | AT rohchanghyun productionofanticanceragentusingmicrobialbiotransformation AT kangchankyu productionofanticanceragentusingmicrobialbiotransformation |