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Complement Fixing Polysaccharides from Terminalia macroptera Root Bark, Stem Bark and Leaves

The root bark, stem bark and leaves of Terminalia macroptera were sequentially extracted with ethanol, 50% ethanol-water, and 50 °C and 100 °C water using an accelerated solvent extractor. Ten bioactive purified polysaccharide fractions were obtained from those crude extracts after anion exchange ch...

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Autores principales: Zou, Yuan-Feng, Zhang, Bing-Zhao, Barsett, Hilde, Inngjerdingen, Kari Tvete, Diallo, Drissa, Michaelsen, Terje Einar, Paulsen, Berit Smestad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270672/
https://www.ncbi.nlm.nih.gov/pubmed/24914893
http://dx.doi.org/10.3390/molecules19067440
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author Zou, Yuan-Feng
Zhang, Bing-Zhao
Barsett, Hilde
Inngjerdingen, Kari Tvete
Diallo, Drissa
Michaelsen, Terje Einar
Paulsen, Berit Smestad
author_facet Zou, Yuan-Feng
Zhang, Bing-Zhao
Barsett, Hilde
Inngjerdingen, Kari Tvete
Diallo, Drissa
Michaelsen, Terje Einar
Paulsen, Berit Smestad
author_sort Zou, Yuan-Feng
collection PubMed
description The root bark, stem bark and leaves of Terminalia macroptera were sequentially extracted with ethanol, 50% ethanol-water, and 50 °C and 100 °C water using an accelerated solvent extractor. Ten bioactive purified polysaccharide fractions were obtained from those crude extracts after anion exchange chromatography and gel filtration. The polysaccharides and their native extracts were characterized with respect to molecular weight, chemical compositions and effects in the complement assay. The chemical compositions showed that the polysaccharides are of pectic nature. The results indicated that there was no great difference of the complement fixation activities in the crude extracts from the different plant parts when extracting with the accelerated solvent extraction system. The purified polysaccharide fractions 100WTSBH-I-I and 100WTRBH-I-I isolated from the 100 °C water extracts of stem and root bark respectively, showed the highest complement fixation activities. These two fractions have rhamnogalacturonan type I backbone, but only 100WTSBH-I-I contains side chains of both arabinogalactan type I and II. Based on the yield and activities of the fractions studied those from the root bark gave highest results, followed by those from leaves and stem bark. But in total, all plant materials are good sources for fractions containing bioactive polysaccharides.
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spelling pubmed-62706722018-12-21 Complement Fixing Polysaccharides from Terminalia macroptera Root Bark, Stem Bark and Leaves Zou, Yuan-Feng Zhang, Bing-Zhao Barsett, Hilde Inngjerdingen, Kari Tvete Diallo, Drissa Michaelsen, Terje Einar Paulsen, Berit Smestad Molecules Article The root bark, stem bark and leaves of Terminalia macroptera were sequentially extracted with ethanol, 50% ethanol-water, and 50 °C and 100 °C water using an accelerated solvent extractor. Ten bioactive purified polysaccharide fractions were obtained from those crude extracts after anion exchange chromatography and gel filtration. The polysaccharides and their native extracts were characterized with respect to molecular weight, chemical compositions and effects in the complement assay. The chemical compositions showed that the polysaccharides are of pectic nature. The results indicated that there was no great difference of the complement fixation activities in the crude extracts from the different plant parts when extracting with the accelerated solvent extraction system. The purified polysaccharide fractions 100WTSBH-I-I and 100WTRBH-I-I isolated from the 100 °C water extracts of stem and root bark respectively, showed the highest complement fixation activities. These two fractions have rhamnogalacturonan type I backbone, but only 100WTSBH-I-I contains side chains of both arabinogalactan type I and II. Based on the yield and activities of the fractions studied those from the root bark gave highest results, followed by those from leaves and stem bark. But in total, all plant materials are good sources for fractions containing bioactive polysaccharides. MDPI 2014-06-06 /pmc/articles/PMC6270672/ /pubmed/24914893 http://dx.doi.org/10.3390/molecules19067440 Text en © 2014 by the authors. licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zou, Yuan-Feng
Zhang, Bing-Zhao
Barsett, Hilde
Inngjerdingen, Kari Tvete
Diallo, Drissa
Michaelsen, Terje Einar
Paulsen, Berit Smestad
Complement Fixing Polysaccharides from Terminalia macroptera Root Bark, Stem Bark and Leaves
title Complement Fixing Polysaccharides from Terminalia macroptera Root Bark, Stem Bark and Leaves
title_full Complement Fixing Polysaccharides from Terminalia macroptera Root Bark, Stem Bark and Leaves
title_fullStr Complement Fixing Polysaccharides from Terminalia macroptera Root Bark, Stem Bark and Leaves
title_full_unstemmed Complement Fixing Polysaccharides from Terminalia macroptera Root Bark, Stem Bark and Leaves
title_short Complement Fixing Polysaccharides from Terminalia macroptera Root Bark, Stem Bark and Leaves
title_sort complement fixing polysaccharides from terminalia macroptera root bark, stem bark and leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270672/
https://www.ncbi.nlm.nih.gov/pubmed/24914893
http://dx.doi.org/10.3390/molecules19067440
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