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Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MS(n)

Danggui Buxue Tang (DBT) is a Traditional Chinese Medicine (TCM) formula primarily used to treat symptoms associated with menopause in women. Usually, DBT is composed of one portion of Radix Angelicae Sinensis (RAS) and five portions of Radix Astragali (RA). Clinically, Radix Hedysari (RH) is someti...

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Autores principales: Zhang, Ya-Zhou, Xu, Feng, Yi, Tao, Zhang, Jian-Ye, Xu, Jun, Tang, Yi-Na, He, Xi-Chen, Liu, Jing, Chen, Hu-Biao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270708/
https://www.ncbi.nlm.nih.gov/pubmed/24786849
http://dx.doi.org/10.3390/molecules19055650
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author Zhang, Ya-Zhou
Xu, Feng
Yi, Tao
Zhang, Jian-Ye
Xu, Jun
Tang, Yi-Na
He, Xi-Chen
Liu, Jing
Chen, Hu-Biao
author_facet Zhang, Ya-Zhou
Xu, Feng
Yi, Tao
Zhang, Jian-Ye
Xu, Jun
Tang, Yi-Na
He, Xi-Chen
Liu, Jing
Chen, Hu-Biao
author_sort Zhang, Ya-Zhou
collection PubMed
description Danggui Buxue Tang (DBT) is a Traditional Chinese Medicine (TCM) formula primarily used to treat symptoms associated with menopause in women. Usually, DBT is composed of one portion of Radix Angelicae Sinensis (RAS) and five portions of Radix Astragali (RA). Clinically, Radix Hedysari (RH) is sometimes used by TCM physicians to replace RA in DBT. In order to verity whether the chemical constituents of the DBT1 (RA:RAS = 5:1, w/w) and DBT2 (RH:RAS = 5:1, w/w) share similarities the chemical profiles of the two DBTs crude extracts and urine samples were analyzed and compared with the aid of HPLC-DAD-ESI-IT-TOF-MS(n), which determines the total ion chromatogram (TIC) and multi-stage mass spectra (MS(n)). Then, the DBT1 and DBT2 were identified and compared on the basis of the TIC and the MS(n). In the first experiment (with crude extracts), 69 compounds (C1–C69) were identified from the DBT1; 46 compounds (c1–c46) were identified from the DBT2. In the second experiment(with urine samples), 44 compounds (M1–M44) were identified from the urine samples of rats that had been administered DBT1, and 34 compounds (m1–m34) were identified from the urine samples of rats that had been administered DBT2. Identification and comparison of the chemical compositions were carried out between the DBT1 and DBT2 of the crude extracts and urine samples respectively. Our results showed that the two crude extracts of the DBTs have quite different chemical profiles. The reasons for their differences were that the special astragalosides in DBT1 and the isoflavonoid glycosides formed the malonic acid esters undergo single esterification and acetyl esters undergo acetylation in DBT1. In contrast, the urine from DBT1-treated rats strongly resembled that of DBT2-treated rats. These metabolites originate mainly from formononetin, calycosin and their related glycosides, and they were formed mainly by the metabolic process of reduction, deglycosylation, demethylation, hydrogenation and sulfation. The HPLC-DAD-ESI-IT-TOF-MS(n) method was successfully applied for the rapid chemical profiles evaluation of two DBTs and their related urine samples.
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spelling pubmed-62707082018-12-21 Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MS(n) Zhang, Ya-Zhou Xu, Feng Yi, Tao Zhang, Jian-Ye Xu, Jun Tang, Yi-Na He, Xi-Chen Liu, Jing Chen, Hu-Biao Molecules Article Danggui Buxue Tang (DBT) is a Traditional Chinese Medicine (TCM) formula primarily used to treat symptoms associated with menopause in women. Usually, DBT is composed of one portion of Radix Angelicae Sinensis (RAS) and five portions of Radix Astragali (RA). Clinically, Radix Hedysari (RH) is sometimes used by TCM physicians to replace RA in DBT. In order to verity whether the chemical constituents of the DBT1 (RA:RAS = 5:1, w/w) and DBT2 (RH:RAS = 5:1, w/w) share similarities the chemical profiles of the two DBTs crude extracts and urine samples were analyzed and compared with the aid of HPLC-DAD-ESI-IT-TOF-MS(n), which determines the total ion chromatogram (TIC) and multi-stage mass spectra (MS(n)). Then, the DBT1 and DBT2 were identified and compared on the basis of the TIC and the MS(n). In the first experiment (with crude extracts), 69 compounds (C1–C69) were identified from the DBT1; 46 compounds (c1–c46) were identified from the DBT2. In the second experiment(with urine samples), 44 compounds (M1–M44) were identified from the urine samples of rats that had been administered DBT1, and 34 compounds (m1–m34) were identified from the urine samples of rats that had been administered DBT2. Identification and comparison of the chemical compositions were carried out between the DBT1 and DBT2 of the crude extracts and urine samples respectively. Our results showed that the two crude extracts of the DBTs have quite different chemical profiles. The reasons for their differences were that the special astragalosides in DBT1 and the isoflavonoid glycosides formed the malonic acid esters undergo single esterification and acetyl esters undergo acetylation in DBT1. In contrast, the urine from DBT1-treated rats strongly resembled that of DBT2-treated rats. These metabolites originate mainly from formononetin, calycosin and their related glycosides, and they were formed mainly by the metabolic process of reduction, deglycosylation, demethylation, hydrogenation and sulfation. The HPLC-DAD-ESI-IT-TOF-MS(n) method was successfully applied for the rapid chemical profiles evaluation of two DBTs and their related urine samples. MDPI 2014-04-30 /pmc/articles/PMC6270708/ /pubmed/24786849 http://dx.doi.org/10.3390/molecules19055650 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhang, Ya-Zhou
Xu, Feng
Yi, Tao
Zhang, Jian-Ye
Xu, Jun
Tang, Yi-Na
He, Xi-Chen
Liu, Jing
Chen, Hu-Biao
Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MS(n)
title Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MS(n)
title_full Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MS(n)
title_fullStr Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MS(n)
title_full_unstemmed Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MS(n)
title_short Chemical Profile Analysis and Comparison of Two Versions of the Classic TCM Formula Danggui Buxue Tang by HPLC-DAD-ESI-IT-TOF-MS(n)
title_sort chemical profile analysis and comparison of two versions of the classic tcm formula danggui buxue tang by hplc-dad-esi-it-tof-ms(n)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270708/
https://www.ncbi.nlm.nih.gov/pubmed/24786849
http://dx.doi.org/10.3390/molecules19055650
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