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Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer

Lung cancer is the major cause of cancer-related deaths worldwide, thus developing effective methods for its early diagnosis is urgently needed. In recent years, microRNAs (miRNAs, miR) have been reported to play important roles in carcinogenesis and have become potential biomarkers for cancer diagn...

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Autores principales: Zhu, Hai-Zhen, An, Jiang-Hong, Yao, Quan, Han, Jing, Li, Xue-Tao, Jiang, Fei-Long, Chen, Guang-Peng, Peng, Li-Na, Li, Yong-Sheng, Sun, Jian-Guo, Chen, Zheng-Tang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270718/
https://www.ncbi.nlm.nih.gov/pubmed/25230125
http://dx.doi.org/10.3390/molecules190914710
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author Zhu, Hai-Zhen
An, Jiang-Hong
Yao, Quan
Han, Jing
Li, Xue-Tao
Jiang, Fei-Long
Chen, Guang-Peng
Peng, Li-Na
Li, Yong-Sheng
Sun, Jian-Guo
Chen, Zheng-Tang
author_facet Zhu, Hai-Zhen
An, Jiang-Hong
Yao, Quan
Han, Jing
Li, Xue-Tao
Jiang, Fei-Long
Chen, Guang-Peng
Peng, Li-Na
Li, Yong-Sheng
Sun, Jian-Guo
Chen, Zheng-Tang
author_sort Zhu, Hai-Zhen
collection PubMed
description Lung cancer is the major cause of cancer-related deaths worldwide, thus developing effective methods for its early diagnosis is urgently needed. In recent years, microRNAs (miRNAs, miR) have been reported to play important roles in carcinogenesis and have become potential biomarkers for cancer diagnosis and treatment. Molecular beacon (MB) technology is a universal technology to detect DNA/RNA expression in living cells. As a natural polymers, chitosan (CS) nanoparticles could be used as a carrier for safe delivery of nucleic acid. In this study, we developed a probe using nanoparticles of miR-155 MB self assembled with CS (CS-miR-155 MB) to image the expression of miR-155 in cancer cells. Hybridization assay showed that the locked nucleic acid (LAN) modified miR-155 MB could target miR-155 effectively and sensitively. The miR-155 MB self-assembly with CS nanoparticles formed stable complexes at the proper weight ratio. The CS nanoparticles showed higher fluorescence intensity and transfection efficiency than the lipid-based formulation transfection agent by confocal microscopy and flow cytometry analysis. The CS-MB complexes were found to be easily synthesized and exhibited strong enzymatic stability, efficient cellular uptake, high target selectivity and biocompatibility. The CS-MB complexes can also be applied in other cancers just by simply changing for a targeted miRNA highly expressed in those cancer cells. Therefore, it is a promising vehicle used for detecting miRNA expression in living cells.
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spelling pubmed-62707182018-12-27 Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer Zhu, Hai-Zhen An, Jiang-Hong Yao, Quan Han, Jing Li, Xue-Tao Jiang, Fei-Long Chen, Guang-Peng Peng, Li-Na Li, Yong-Sheng Sun, Jian-Guo Chen, Zheng-Tang Molecules Article Lung cancer is the major cause of cancer-related deaths worldwide, thus developing effective methods for its early diagnosis is urgently needed. In recent years, microRNAs (miRNAs, miR) have been reported to play important roles in carcinogenesis and have become potential biomarkers for cancer diagnosis and treatment. Molecular beacon (MB) technology is a universal technology to detect DNA/RNA expression in living cells. As a natural polymers, chitosan (CS) nanoparticles could be used as a carrier for safe delivery of nucleic acid. In this study, we developed a probe using nanoparticles of miR-155 MB self assembled with CS (CS-miR-155 MB) to image the expression of miR-155 in cancer cells. Hybridization assay showed that the locked nucleic acid (LAN) modified miR-155 MB could target miR-155 effectively and sensitively. The miR-155 MB self-assembly with CS nanoparticles formed stable complexes at the proper weight ratio. The CS nanoparticles showed higher fluorescence intensity and transfection efficiency than the lipid-based formulation transfection agent by confocal microscopy and flow cytometry analysis. The CS-MB complexes were found to be easily synthesized and exhibited strong enzymatic stability, efficient cellular uptake, high target selectivity and biocompatibility. The CS-MB complexes can also be applied in other cancers just by simply changing for a targeted miRNA highly expressed in those cancer cells. Therefore, it is a promising vehicle used for detecting miRNA expression in living cells. MDPI 2014-09-16 /pmc/articles/PMC6270718/ /pubmed/25230125 http://dx.doi.org/10.3390/molecules190914710 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhu, Hai-Zhen
An, Jiang-Hong
Yao, Quan
Han, Jing
Li, Xue-Tao
Jiang, Fei-Long
Chen, Guang-Peng
Peng, Li-Na
Li, Yong-Sheng
Sun, Jian-Guo
Chen, Zheng-Tang
Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer
title Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer
title_full Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer
title_fullStr Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer
title_full_unstemmed Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer
title_short Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer
title_sort chitosan combined with molecular beacon for mir-155 detection and imaging in lung cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270718/
https://www.ncbi.nlm.nih.gov/pubmed/25230125
http://dx.doi.org/10.3390/molecules190914710
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