Formation of a Flavin-Linked Peptide

In a previous study, we showed that formylmethylflavin (FMF) can bind to cysteine. In this study, FMF was reacted with native peptides (CG and CKLVFF) containing an N-terminal cysteine. The formation of flavin-CG and flavin-CKLVFF was confirmed using HPLC and ESI-MS. Storage of flavin-CKLVFF in DMSO...

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Detalles Bibliográficos
Autores principales: Morikawa, Masayuki, Kino, Katsuhito, Senda, Takeshi, Suzuki, Masayo, Kobayashi, Takanobu, Miyazawa, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270736/
https://www.ncbi.nlm.nih.gov/pubmed/25000466
http://dx.doi.org/10.3390/molecules19079552
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author Morikawa, Masayuki
Kino, Katsuhito
Senda, Takeshi
Suzuki, Masayo
Kobayashi, Takanobu
Miyazawa, Hiroshi
author_facet Morikawa, Masayuki
Kino, Katsuhito
Senda, Takeshi
Suzuki, Masayo
Kobayashi, Takanobu
Miyazawa, Hiroshi
author_sort Morikawa, Masayuki
collection PubMed
description In a previous study, we showed that formylmethylflavin (FMF) can bind to cysteine. In this study, FMF was reacted with native peptides (CG and CKLVFF) containing an N-terminal cysteine. The formation of flavin-CG and flavin-CKLVFF was confirmed using HPLC and ESI-MS. Storage of flavin-CKLVFF in DMSO at −30 °C for 7 days resulted in no detectable deposition. In contrast, flavin-CKLVFF formed deposits when stored in water at −30 °C for 1 day, but no deposit was observed in the aqueous solution of flavin-CKLVFF after 7 days storage in the presence of 0.1% Triton X-100.
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spelling pubmed-62707362018-12-21 Formation of a Flavin-Linked Peptide Morikawa, Masayuki Kino, Katsuhito Senda, Takeshi Suzuki, Masayo Kobayashi, Takanobu Miyazawa, Hiroshi Molecules Article In a previous study, we showed that formylmethylflavin (FMF) can bind to cysteine. In this study, FMF was reacted with native peptides (CG and CKLVFF) containing an N-terminal cysteine. The formation of flavin-CG and flavin-CKLVFF was confirmed using HPLC and ESI-MS. Storage of flavin-CKLVFF in DMSO at −30 °C for 7 days resulted in no detectable deposition. In contrast, flavin-CKLVFF formed deposits when stored in water at −30 °C for 1 day, but no deposit was observed in the aqueous solution of flavin-CKLVFF after 7 days storage in the presence of 0.1% Triton X-100. MDPI 2014-07-04 /pmc/articles/PMC6270736/ /pubmed/25000466 http://dx.doi.org/10.3390/molecules19079552 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Morikawa, Masayuki
Kino, Katsuhito
Senda, Takeshi
Suzuki, Masayo
Kobayashi, Takanobu
Miyazawa, Hiroshi
Formation of a Flavin-Linked Peptide
title Formation of a Flavin-Linked Peptide
title_full Formation of a Flavin-Linked Peptide
title_fullStr Formation of a Flavin-Linked Peptide
title_full_unstemmed Formation of a Flavin-Linked Peptide
title_short Formation of a Flavin-Linked Peptide
title_sort formation of a flavin-linked peptide
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270736/
https://www.ncbi.nlm.nih.gov/pubmed/25000466
http://dx.doi.org/10.3390/molecules19079552
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AT kobayashitakanobu formationofaflavinlinkedpeptide
AT miyazawahiroshi formationofaflavinlinkedpeptide

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