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Biotransformation and in Vitro Metabolic Profile of Bioactive Extracts from a Traditional Miao-Nationality Herbal Medicine, Polygonum capitatum

Polygonum capitatum Buch.-Ham.ex D. Don, a traditional Miao-nationality herbal medicine, has been widely used in the treatment of various urologic disorders. Recent pharmacological studies demonstrated that a pure compound, FR429, isolated from the ethanol extracts of P. capitatum could selectively...

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Autores principales: He, Chi-Yu, Fu, Jie, Ma, Jing-Yi, Feng, Ru, Tan, Xiang-Shan, Huang, Min, Shou, Jia-Wen, Zhao, Zhen-Xiong, Li, Xiao-Yang, Zhang, Xian-Feng, Chen, Yangchao, Wang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270779/
https://www.ncbi.nlm.nih.gov/pubmed/25033057
http://dx.doi.org/10.3390/molecules190710291
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author He, Chi-Yu
Fu, Jie
Ma, Jing-Yi
Feng, Ru
Tan, Xiang-Shan
Huang, Min
Shou, Jia-Wen
Zhao, Zhen-Xiong
Li, Xiao-Yang
Zhang, Xian-Feng
Chen, Yangchao
Wang, Yan
author_facet He, Chi-Yu
Fu, Jie
Ma, Jing-Yi
Feng, Ru
Tan, Xiang-Shan
Huang, Min
Shou, Jia-Wen
Zhao, Zhen-Xiong
Li, Xiao-Yang
Zhang, Xian-Feng
Chen, Yangchao
Wang, Yan
author_sort He, Chi-Yu
collection PubMed
description Polygonum capitatum Buch.-Ham.ex D. Don, a traditional Miao-nationality herbal medicine, has been widely used in the treatment of various urologic disorders. Recent pharmacological studies demonstrated that a pure compound, FR429, isolated from the ethanol extracts of P. capitatum could selectively inhibit the growth of four hepatocellular carcinoma (HCC) cell lines in a dose-dependent manner. Thus, P. capitatum probably exhibits potential antitumor activity. However, there is very little information on the metabolism of substances present in P. capitatum extracts. In this study, gallic acid, quercetrin, ethanol extracts and ethyl acetate fraction of ethnolic extract (EtOAc fraction) of P. capitatum were cultured anaerobically with rat intestinal bacteria. A highly sensitive and selective liquid chromatography electrospray ionization-ion trap-time of fight mass spectrometry (LC/MS(n)-IT-TOF) technique was employed to identify and characterize the resulting metabolites. A total of 22 metabolites (M1–M22), including tannins, phenolic acids and flavonoids, were detected and characterized. The overall results demonstrated that the intestinal bacteria played an important role in the metabolism of P. capitatum, and the main metabolic pathways were hydrolysis, reduction and oxidation reactions. Our results provided a basis for the estimation of the metabolic transformation of P. capitatum in vivo.
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spelling pubmed-62707792018-12-21 Biotransformation and in Vitro Metabolic Profile of Bioactive Extracts from a Traditional Miao-Nationality Herbal Medicine, Polygonum capitatum He, Chi-Yu Fu, Jie Ma, Jing-Yi Feng, Ru Tan, Xiang-Shan Huang, Min Shou, Jia-Wen Zhao, Zhen-Xiong Li, Xiao-Yang Zhang, Xian-Feng Chen, Yangchao Wang, Yan Molecules Article Polygonum capitatum Buch.-Ham.ex D. Don, a traditional Miao-nationality herbal medicine, has been widely used in the treatment of various urologic disorders. Recent pharmacological studies demonstrated that a pure compound, FR429, isolated from the ethanol extracts of P. capitatum could selectively inhibit the growth of four hepatocellular carcinoma (HCC) cell lines in a dose-dependent manner. Thus, P. capitatum probably exhibits potential antitumor activity. However, there is very little information on the metabolism of substances present in P. capitatum extracts. In this study, gallic acid, quercetrin, ethanol extracts and ethyl acetate fraction of ethnolic extract (EtOAc fraction) of P. capitatum were cultured anaerobically with rat intestinal bacteria. A highly sensitive and selective liquid chromatography electrospray ionization-ion trap-time of fight mass spectrometry (LC/MS(n)-IT-TOF) technique was employed to identify and characterize the resulting metabolites. A total of 22 metabolites (M1–M22), including tannins, phenolic acids and flavonoids, were detected and characterized. The overall results demonstrated that the intestinal bacteria played an important role in the metabolism of P. capitatum, and the main metabolic pathways were hydrolysis, reduction and oxidation reactions. Our results provided a basis for the estimation of the metabolic transformation of P. capitatum in vivo. MDPI 2014-07-16 /pmc/articles/PMC6270779/ /pubmed/25033057 http://dx.doi.org/10.3390/molecules190710291 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Chi-Yu
Fu, Jie
Ma, Jing-Yi
Feng, Ru
Tan, Xiang-Shan
Huang, Min
Shou, Jia-Wen
Zhao, Zhen-Xiong
Li, Xiao-Yang
Zhang, Xian-Feng
Chen, Yangchao
Wang, Yan
Biotransformation and in Vitro Metabolic Profile of Bioactive Extracts from a Traditional Miao-Nationality Herbal Medicine, Polygonum capitatum
title Biotransformation and in Vitro Metabolic Profile of Bioactive Extracts from a Traditional Miao-Nationality Herbal Medicine, Polygonum capitatum
title_full Biotransformation and in Vitro Metabolic Profile of Bioactive Extracts from a Traditional Miao-Nationality Herbal Medicine, Polygonum capitatum
title_fullStr Biotransformation and in Vitro Metabolic Profile of Bioactive Extracts from a Traditional Miao-Nationality Herbal Medicine, Polygonum capitatum
title_full_unstemmed Biotransformation and in Vitro Metabolic Profile of Bioactive Extracts from a Traditional Miao-Nationality Herbal Medicine, Polygonum capitatum
title_short Biotransformation and in Vitro Metabolic Profile of Bioactive Extracts from a Traditional Miao-Nationality Herbal Medicine, Polygonum capitatum
title_sort biotransformation and in vitro metabolic profile of bioactive extracts from a traditional miao-nationality herbal medicine, polygonum capitatum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270779/
https://www.ncbi.nlm.nih.gov/pubmed/25033057
http://dx.doi.org/10.3390/molecules190710291
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