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Hepatoprotective Effects of Met-enkephalin on Acetaminophen-Induced Liver Lesions in Male CBA Mice
Recent histopathological investigations in patients with hepatitis suggested possible involvement of Met-enkephalin and its receptors in the pathophysiology of hepatitis. Consequently, we evaluated the potential hepatoprotective effects of this endogenous opioid pentapeptide in the experimental mode...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270943/ https://www.ncbi.nlm.nih.gov/pubmed/25105920 http://dx.doi.org/10.3390/molecules190811833 |
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author | Martinić, Roko Šošić, Hrvoje Turčić, Petra Konjevoda, Paško Fučić, Aleksandra Stojković, Ranko Aralica, Gorana Gabričević, Mario Weitner, Tin Štambuk, Nikola |
author_facet | Martinić, Roko Šošić, Hrvoje Turčić, Petra Konjevoda, Paško Fučić, Aleksandra Stojković, Ranko Aralica, Gorana Gabričević, Mario Weitner, Tin Štambuk, Nikola |
author_sort | Martinić, Roko |
collection | PubMed |
description | Recent histopathological investigations in patients with hepatitis suggested possible involvement of Met-enkephalin and its receptors in the pathophysiology of hepatitis. Consequently, we evaluated the potential hepatoprotective effects of this endogenous opioid pentapeptide in the experimental model of acetaminophen induced hepatotoxicity in male CBA mice. Met-enkephalin exhibited strong hepatoprotective effects in a dose of 7.5 mg/kg, which corresponds to the protective dose reported for several different animal disease models. In this group plasma alanine aminotransferase and aspartate aminotransferase enzyme activities, as well as liver necrosis score were significantly reduced in comparison to control animals treated with physiological saline (p > 0.01). The specificity of the peptide hepatoprotection was investigated from the standpoint of the receptor and peptide blockade. It was concluded that Met-enkephalin effects on the liver were mediated via δ and ζ opioid receptors. Genotoxic testing of Met-enkephalin confirmed the safety of the peptide. |
format | Online Article Text |
id | pubmed-6270943 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62709432018-12-27 Hepatoprotective Effects of Met-enkephalin on Acetaminophen-Induced Liver Lesions in Male CBA Mice Martinić, Roko Šošić, Hrvoje Turčić, Petra Konjevoda, Paško Fučić, Aleksandra Stojković, Ranko Aralica, Gorana Gabričević, Mario Weitner, Tin Štambuk, Nikola Molecules Article Recent histopathological investigations in patients with hepatitis suggested possible involvement of Met-enkephalin and its receptors in the pathophysiology of hepatitis. Consequently, we evaluated the potential hepatoprotective effects of this endogenous opioid pentapeptide in the experimental model of acetaminophen induced hepatotoxicity in male CBA mice. Met-enkephalin exhibited strong hepatoprotective effects in a dose of 7.5 mg/kg, which corresponds to the protective dose reported for several different animal disease models. In this group plasma alanine aminotransferase and aspartate aminotransferase enzyme activities, as well as liver necrosis score were significantly reduced in comparison to control animals treated with physiological saline (p > 0.01). The specificity of the peptide hepatoprotection was investigated from the standpoint of the receptor and peptide blockade. It was concluded that Met-enkephalin effects on the liver were mediated via δ and ζ opioid receptors. Genotoxic testing of Met-enkephalin confirmed the safety of the peptide. MDPI 2014-08-07 /pmc/articles/PMC6270943/ /pubmed/25105920 http://dx.doi.org/10.3390/molecules190811833 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Martinić, Roko Šošić, Hrvoje Turčić, Petra Konjevoda, Paško Fučić, Aleksandra Stojković, Ranko Aralica, Gorana Gabričević, Mario Weitner, Tin Štambuk, Nikola Hepatoprotective Effects of Met-enkephalin on Acetaminophen-Induced Liver Lesions in Male CBA Mice |
title | Hepatoprotective Effects of Met-enkephalin on Acetaminophen-Induced Liver Lesions in Male CBA Mice |
title_full | Hepatoprotective Effects of Met-enkephalin on Acetaminophen-Induced Liver Lesions in Male CBA Mice |
title_fullStr | Hepatoprotective Effects of Met-enkephalin on Acetaminophen-Induced Liver Lesions in Male CBA Mice |
title_full_unstemmed | Hepatoprotective Effects of Met-enkephalin on Acetaminophen-Induced Liver Lesions in Male CBA Mice |
title_short | Hepatoprotective Effects of Met-enkephalin on Acetaminophen-Induced Liver Lesions in Male CBA Mice |
title_sort | hepatoprotective effects of met-enkephalin on acetaminophen-induced liver lesions in male cba mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270943/ https://www.ncbi.nlm.nih.gov/pubmed/25105920 http://dx.doi.org/10.3390/molecules190811833 |
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