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Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola

Brassica species produce the antifungal indolyl compounds brassinin and its derivatives, during microbial infection. The fungal pathogen Alternaria brassicicola detoxifies brassinin and possibly its derivatives. This ability is an important property for the successful infection of brassicaceous plan...

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Autores principales: Cho, Yangrae, Ohm, Robin A., Devappa, Rakshit, Lee, Hyang Burm, Grigoriev, Igor V., Kim, Bo Yeon, Ahn, Jong Seog
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270968/
https://www.ncbi.nlm.nih.gov/pubmed/25061722
http://dx.doi.org/10.3390/molecules190810717
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author Cho, Yangrae
Ohm, Robin A.
Devappa, Rakshit
Lee, Hyang Burm
Grigoriev, Igor V.
Kim, Bo Yeon
Ahn, Jong Seog
author_facet Cho, Yangrae
Ohm, Robin A.
Devappa, Rakshit
Lee, Hyang Burm
Grigoriev, Igor V.
Kim, Bo Yeon
Ahn, Jong Seog
author_sort Cho, Yangrae
collection PubMed
description Brassica species produce the antifungal indolyl compounds brassinin and its derivatives, during microbial infection. The fungal pathogen Alternaria brassicicola detoxifies brassinin and possibly its derivatives. This ability is an important property for the successful infection of brassicaceous plants. Previously, we identified a transcription factor, Bdtf1, essential for the detoxification of brassinin and full virulence. To discover genes that encode putative brassinin-digesting enzymes, we compared gene expression profiles between a mutant strain of the transcription factor and wild-type A. brassicicola under two different experimental conditions. A total of 170 and 388 genes were expressed at higher levels in the mutants than the wild type during the infection of host plants and saprophytic growth in the presence of brassinin, respectively. In contrast, 93 and 560 genes were expressed, respectively, at lower levels in the mutant than the wild type under the two conditions. Fifteen of these genes were expressed at lower levels in the mutant than in the wild type under both conditions. These genes were assumed to be important for the detoxification of brassinin and included Bdtf1 and 10 putative enzymes. This list of genes provides a resource for the discovery of enzyme-coding genes important in the chemical modification of brassinin.
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spelling pubmed-62709682018-12-27 Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola Cho, Yangrae Ohm, Robin A. Devappa, Rakshit Lee, Hyang Burm Grigoriev, Igor V. Kim, Bo Yeon Ahn, Jong Seog Molecules Article Brassica species produce the antifungal indolyl compounds brassinin and its derivatives, during microbial infection. The fungal pathogen Alternaria brassicicola detoxifies brassinin and possibly its derivatives. This ability is an important property for the successful infection of brassicaceous plants. Previously, we identified a transcription factor, Bdtf1, essential for the detoxification of brassinin and full virulence. To discover genes that encode putative brassinin-digesting enzymes, we compared gene expression profiles between a mutant strain of the transcription factor and wild-type A. brassicicola under two different experimental conditions. A total of 170 and 388 genes were expressed at higher levels in the mutants than the wild type during the infection of host plants and saprophytic growth in the presence of brassinin, respectively. In contrast, 93 and 560 genes were expressed, respectively, at lower levels in the mutant than the wild type under the two conditions. Fifteen of these genes were expressed at lower levels in the mutant than in the wild type under both conditions. These genes were assumed to be important for the detoxification of brassinin and included Bdtf1 and 10 putative enzymes. This list of genes provides a resource for the discovery of enzyme-coding genes important in the chemical modification of brassinin. MDPI 2014-07-24 /pmc/articles/PMC6270968/ /pubmed/25061722 http://dx.doi.org/10.3390/molecules190810717 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Cho, Yangrae
Ohm, Robin A.
Devappa, Rakshit
Lee, Hyang Burm
Grigoriev, Igor V.
Kim, Bo Yeon
Ahn, Jong Seog
Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola
title Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola
title_full Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola
title_fullStr Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola
title_full_unstemmed Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola
title_short Transcriptional Responses of the Bdtf1-Deletion Mutant to the Phytoalexin Brassinin in the Necrotrophic Fungus Alternaria brassicicola
title_sort transcriptional responses of the bdtf1-deletion mutant to the phytoalexin brassinin in the necrotrophic fungus alternaria brassicicola
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6270968/
https://www.ncbi.nlm.nih.gov/pubmed/25061722
http://dx.doi.org/10.3390/molecules190810717
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