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Bufalin Alters Gene Expressions Associated DNA Damage, Cell Cycle, and Apoptosis in Human Lung Cancer NCI-H460 Cells in Vitro

Lung cancer is the leading cause of cancer related death and there is no effective treatment to date. Bufalin has been shown effective in inducing apoptosis and DNA damage in lung cancer cells. However, the genetic mechanisms underlying these actions have not been elucidated yet. Cultured NCI-H460 c...

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Autores principales: Wu, Shin-Hwar, Hsiao, Yung-Ting, Chen, Jaw-Chyum, Lin, Ju-Hwa, Hsu, Shu-Chun, Hsia, Te-Chun, Yang, Su-Tso, Hsu, Wu-Huei, Chung, Jing-Gung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271037/
https://www.ncbi.nlm.nih.gov/pubmed/24828377
http://dx.doi.org/10.3390/molecules19056047
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author Wu, Shin-Hwar
Hsiao, Yung-Ting
Chen, Jaw-Chyum
Lin, Ju-Hwa
Hsu, Shu-Chun
Hsia, Te-Chun
Yang, Su-Tso
Hsu, Wu-Huei
Chung, Jing-Gung
author_facet Wu, Shin-Hwar
Hsiao, Yung-Ting
Chen, Jaw-Chyum
Lin, Ju-Hwa
Hsu, Shu-Chun
Hsia, Te-Chun
Yang, Su-Tso
Hsu, Wu-Huei
Chung, Jing-Gung
author_sort Wu, Shin-Hwar
collection PubMed
description Lung cancer is the leading cause of cancer related death and there is no effective treatment to date. Bufalin has been shown effective in inducing apoptosis and DNA damage in lung cancer cells. However, the genetic mechanisms underlying these actions have not been elucidated yet. Cultured NCI-H460 cells were treated with or without 2 μM of bufalin for 24 h. The total RNA was extracted from each treatment for cDNA synthesis and labeling, microarray hybridization, and then followed by flour-labeled cDNA hybridized on chip. The localized concentrations of fluorescent molecules were detected and quantitated and analyzed by Expression Console software (Affymetrix) with default RMA parameters. The key genes involved and their possible interaction pathways were mapped by GeneGo software. About 165 apoptosis-related genes were affected. CASP9 was up-regulated by 5.51 fold and THAP1 by 2.75-fold while CCAR1 was down-regulated by 2.24 fold. 107 genes related to DNA damage/repair were affected. MDC1 was down-regulated by 2.22-fold, DDIT4 by 2.52 fold while GADD45B up-regulated by 3.72 fold. 201 genes related to cell cycles were affected. CCPG1 was down-regulated by 2.11 fold and CDCA7L by 2.71 fold. Many genes about apoptosis, cell cycle regulation and DNA repair are changed significantly following bufalin treatment in NCI-H460 cells. These changes provide an in depth understanding of cytotoxic mechanism of bufalin in genetic level and also offer many potentially useful biomarkers for diagnosis and treatment of lung cancer in future.
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spelling pubmed-62710372018-12-21 Bufalin Alters Gene Expressions Associated DNA Damage, Cell Cycle, and Apoptosis in Human Lung Cancer NCI-H460 Cells in Vitro Wu, Shin-Hwar Hsiao, Yung-Ting Chen, Jaw-Chyum Lin, Ju-Hwa Hsu, Shu-Chun Hsia, Te-Chun Yang, Su-Tso Hsu, Wu-Huei Chung, Jing-Gung Molecules Article Lung cancer is the leading cause of cancer related death and there is no effective treatment to date. Bufalin has been shown effective in inducing apoptosis and DNA damage in lung cancer cells. However, the genetic mechanisms underlying these actions have not been elucidated yet. Cultured NCI-H460 cells were treated with or without 2 μM of bufalin for 24 h. The total RNA was extracted from each treatment for cDNA synthesis and labeling, microarray hybridization, and then followed by flour-labeled cDNA hybridized on chip. The localized concentrations of fluorescent molecules were detected and quantitated and analyzed by Expression Console software (Affymetrix) with default RMA parameters. The key genes involved and their possible interaction pathways were mapped by GeneGo software. About 165 apoptosis-related genes were affected. CASP9 was up-regulated by 5.51 fold and THAP1 by 2.75-fold while CCAR1 was down-regulated by 2.24 fold. 107 genes related to DNA damage/repair were affected. MDC1 was down-regulated by 2.22-fold, DDIT4 by 2.52 fold while GADD45B up-regulated by 3.72 fold. 201 genes related to cell cycles were affected. CCPG1 was down-regulated by 2.11 fold and CDCA7L by 2.71 fold. Many genes about apoptosis, cell cycle regulation and DNA repair are changed significantly following bufalin treatment in NCI-H460 cells. These changes provide an in depth understanding of cytotoxic mechanism of bufalin in genetic level and also offer many potentially useful biomarkers for diagnosis and treatment of lung cancer in future. MDPI 2014-05-13 /pmc/articles/PMC6271037/ /pubmed/24828377 http://dx.doi.org/10.3390/molecules19056047 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Wu, Shin-Hwar
Hsiao, Yung-Ting
Chen, Jaw-Chyum
Lin, Ju-Hwa
Hsu, Shu-Chun
Hsia, Te-Chun
Yang, Su-Tso
Hsu, Wu-Huei
Chung, Jing-Gung
Bufalin Alters Gene Expressions Associated DNA Damage, Cell Cycle, and Apoptosis in Human Lung Cancer NCI-H460 Cells in Vitro
title Bufalin Alters Gene Expressions Associated DNA Damage, Cell Cycle, and Apoptosis in Human Lung Cancer NCI-H460 Cells in Vitro
title_full Bufalin Alters Gene Expressions Associated DNA Damage, Cell Cycle, and Apoptosis in Human Lung Cancer NCI-H460 Cells in Vitro
title_fullStr Bufalin Alters Gene Expressions Associated DNA Damage, Cell Cycle, and Apoptosis in Human Lung Cancer NCI-H460 Cells in Vitro
title_full_unstemmed Bufalin Alters Gene Expressions Associated DNA Damage, Cell Cycle, and Apoptosis in Human Lung Cancer NCI-H460 Cells in Vitro
title_short Bufalin Alters Gene Expressions Associated DNA Damage, Cell Cycle, and Apoptosis in Human Lung Cancer NCI-H460 Cells in Vitro
title_sort bufalin alters gene expressions associated dna damage, cell cycle, and apoptosis in human lung cancer nci-h460 cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271037/
https://www.ncbi.nlm.nih.gov/pubmed/24828377
http://dx.doi.org/10.3390/molecules19056047
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