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Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB

It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of but...

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Autores principales: Alves, Joana S., Garcia-Galan, Cristina, Schein, Mirela F., Silva, Alexandre M., Barbosa, Oveimar, Ayub, Marco A. Z., Fernandez-Lafuente, Roberto, Rodrigues, Rafael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271129/
https://www.ncbi.nlm.nih.gov/pubmed/25004067
http://dx.doi.org/10.3390/molecules19079562
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author Alves, Joana S.
Garcia-Galan, Cristina
Schein, Mirela F.
Silva, Alexandre M.
Barbosa, Oveimar
Ayub, Marco A. Z.
Fernandez-Lafuente, Roberto
Rodrigues, Rafael C.
author_facet Alves, Joana S.
Garcia-Galan, Cristina
Schein, Mirela F.
Silva, Alexandre M.
Barbosa, Oveimar
Ayub, Marco A. Z.
Fernandez-Lafuente, Roberto
Rodrigues, Rafael C.
author_sort Alves, Joana S.
collection PubMed
description It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol:acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system.
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spelling pubmed-62711292018-12-21 Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB Alves, Joana S. Garcia-Galan, Cristina Schein, Mirela F. Silva, Alexandre M. Barbosa, Oveimar Ayub, Marco A. Z. Fernandez-Lafuente, Roberto Rodrigues, Rafael C. Molecules Article It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol:acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system. MDPI 2014-07-07 /pmc/articles/PMC6271129/ /pubmed/25004067 http://dx.doi.org/10.3390/molecules19079562 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alves, Joana S.
Garcia-Galan, Cristina
Schein, Mirela F.
Silva, Alexandre M.
Barbosa, Oveimar
Ayub, Marco A. Z.
Fernandez-Lafuente, Roberto
Rodrigues, Rafael C.
Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
title Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
title_full Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
title_fullStr Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
title_full_unstemmed Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
title_short Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
title_sort combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by calb
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271129/
https://www.ncbi.nlm.nih.gov/pubmed/25004067
http://dx.doi.org/10.3390/molecules19079562
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