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Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB
It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of but...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271129/ https://www.ncbi.nlm.nih.gov/pubmed/25004067 http://dx.doi.org/10.3390/molecules19079562 |
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author | Alves, Joana S. Garcia-Galan, Cristina Schein, Mirela F. Silva, Alexandre M. Barbosa, Oveimar Ayub, Marco A. Z. Fernandez-Lafuente, Roberto Rodrigues, Rafael C. |
author_facet | Alves, Joana S. Garcia-Galan, Cristina Schein, Mirela F. Silva, Alexandre M. Barbosa, Oveimar Ayub, Marco A. Z. Fernandez-Lafuente, Roberto Rodrigues, Rafael C. |
author_sort | Alves, Joana S. |
collection | PubMed |
description | It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol:acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system. |
format | Online Article Text |
id | pubmed-6271129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62711292018-12-21 Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB Alves, Joana S. Garcia-Galan, Cristina Schein, Mirela F. Silva, Alexandre M. Barbosa, Oveimar Ayub, Marco A. Z. Fernandez-Lafuente, Roberto Rodrigues, Rafael C. Molecules Article It is well established that the performance of lipase B from Candida antarctica (CALB) as catalyst for esterification reactions may be improved by the use of ultrasound technology or by its immobilization on styrene-divinylbenzene beads (MCI-CALB). The present research evaluated the synthesis of butyl acetate using MCI-CALB under ultrasonic energy, comparing the results against those obtained using the commercial preparation, Novozym 435. The optimal conditions were determined using response surface methodology (RSM) evaluating the following parameters: reaction temperature, substrate molar ratio, amount of biocatalyst, and added water. The optimal conditions for butyl acetate synthesis catalyzed by MCI-CALB were: temperature, 48.8 °C; substrate molar ratio, 3.46:1 alcohol:acid; amount of biocatalyst, 7.5%; and added water 0.28%, both as substrate mass. Under these conditions, 90% of conversion was reached in 1.5 h. In terms of operational stability, MCI-CALB was reused in seven cycles while keeping 70% of its initial activity under ultrasonic energy. The support pore size and resistance are key points for the enzyme activity and stability under mechanical stirring. The use of ultrasound improved both activity and stability because of better homogeneity and reduced mechanical stress to the immobilized system. MDPI 2014-07-07 /pmc/articles/PMC6271129/ /pubmed/25004067 http://dx.doi.org/10.3390/molecules19079562 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alves, Joana S. Garcia-Galan, Cristina Schein, Mirela F. Silva, Alexandre M. Barbosa, Oveimar Ayub, Marco A. Z. Fernandez-Lafuente, Roberto Rodrigues, Rafael C. Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title | Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_full | Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_fullStr | Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_full_unstemmed | Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_short | Combined Effects of Ultrasound and Immobilization Protocol on Butyl Acetate Synthesis Catalyzed by CALB |
title_sort | combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by calb |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271129/ https://www.ncbi.nlm.nih.gov/pubmed/25004067 http://dx.doi.org/10.3390/molecules19079562 |
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