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Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes
Since the pioneering work of Hirschfeld, it is known that time-integrated emission (TiEm) of a fluorophore is independent of fluorescence quantum yield and illumination intensity. Practical implementation of this important result for determining exact probe distribution in living cells is often hamp...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271172/ https://www.ncbi.nlm.nih.gov/pubmed/25076144 http://dx.doi.org/10.3390/molecules190811096 |