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Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases

The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properti...

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Autores principales: Guzik, Urszula, Hupert-Kocurek, Katarzyna, Wojcieszyńska, Danuta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271243/
https://www.ncbi.nlm.nih.gov/pubmed/24979403
http://dx.doi.org/10.3390/molecules19078995
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author Guzik, Urszula
Hupert-Kocurek, Katarzyna
Wojcieszyńska, Danuta
author_facet Guzik, Urszula
Hupert-Kocurek, Katarzyna
Wojcieszyńska, Danuta
author_sort Guzik, Urszula
collection PubMed
description The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functional stability. Increasing the structural rigidity of the protein and stabilization of multimeric enzymes which prevents dissociation-related inactivation. In the last decade, several papers about immobilization methods have been published. In our work, we present a relation between the influence of immobilization on the improvement of the properties of selected oxidoreductases and their commercial value. We also present our view on the role that different immobilization methods play in the reduction of enzyme inhibition during biotechnological processes.
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spelling pubmed-62712432018-12-21 Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases Guzik, Urszula Hupert-Kocurek, Katarzyna Wojcieszyńska, Danuta Molecules Review The main objective of the immobilization of enzymes is to enhance the economics of biocatalytic processes. Immobilization allows one to re-use the enzyme for an extended period of time and enables easier separation of the catalyst from the product. Additionally, immobilization improves many properties of enzymes such as performance in organic solvents, pH tolerance, heat stability or the functional stability. Increasing the structural rigidity of the protein and stabilization of multimeric enzymes which prevents dissociation-related inactivation. In the last decade, several papers about immobilization methods have been published. In our work, we present a relation between the influence of immobilization on the improvement of the properties of selected oxidoreductases and their commercial value. We also present our view on the role that different immobilization methods play in the reduction of enzyme inhibition during biotechnological processes. MDPI 2014-06-27 /pmc/articles/PMC6271243/ /pubmed/24979403 http://dx.doi.org/10.3390/molecules19078995 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Guzik, Urszula
Hupert-Kocurek, Katarzyna
Wojcieszyńska, Danuta
Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases
title Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases
title_full Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases
title_fullStr Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases
title_full_unstemmed Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases
title_short Immobilization as a Strategy for Improving Enzyme Properties-Application to Oxidoreductases
title_sort immobilization as a strategy for improving enzyme properties-application to oxidoreductases
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271243/
https://www.ncbi.nlm.nih.gov/pubmed/24979403
http://dx.doi.org/10.3390/molecules19078995
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