A Pair of New Antioxidant Phenolic Acid Stereoisomers Isolated from Danshen Injection (Lyophilized Powder)

A pair of new phenolic acid stereoisomers, (R)-norsalvianolic acid L (1) and (S)-norsalvianolic acid L (2), was isolated from the Danshen Injection (lyophilized powder). The structural elucidation and stereochemistry determination were achieved by spectroscopic and chemical methods including 1D, 2D...

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Detalles Bibliográficos
Autores principales: Liu, Jing, Zhao, Jianfeng, Dai, Zhong, Lin, Ruichao, Wang, Gangli, Ma, Shuangcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271289/
https://www.ncbi.nlm.nih.gov/pubmed/24500008
http://dx.doi.org/10.3390/molecules19021786
Descripción
Sumario:A pair of new phenolic acid stereoisomers, (R)-norsalvianolic acid L (1) and (S)-norsalvianolic acid L (2), was isolated from the Danshen Injection (lyophilized powder). The structural elucidation and stereochemistry determination were achieved by spectroscopic and chemical methods including 1D, 2D NMR ((1)H-(1)H COSY, HSQC and HMBC) and circular dichroism experiments. Their antioxidant activities were assessed by the DPPH(·) and ABTS(·+) scavenging methods in vitro with microplate assay. The IC(50) values of 1 were 6.9 and 9.7 μM respectively, which was close to the control salvianolic acid B (7.8 and 7.1 μM respectively), while the IC(50) values of isomer 2 were 27.1 and 25.3 μM, respectively.

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