Cargando…
Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases
A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL(®) CHP20P, has been compared to octyl-Sepharose(®) beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase(®) Ultra, a commercial artifici...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271320/ https://www.ncbi.nlm.nih.gov/pubmed/24918537 http://dx.doi.org/10.3390/molecules19067629 |
Sumario: | A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL(®) CHP20P, has been compared to octyl-Sepharose(®) beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase(®) Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL(®) CHP20P compared to octyl-Sepharose(®) (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase(®) Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose(®) immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose(®) immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL(®) CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones. |
---|