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Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury
Ginseng, the root of Panax ginseng C.A. Meyer, has been used as a tonic to enhance bodily functions against various ailments for hundreds of years in Far Eastern countries without apparent adverse effects. Ginsenoside Rb1, one of the most active ingredients of ginseng, has been shown to possess vari...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271345/ https://www.ncbi.nlm.nih.gov/pubmed/24662068 http://dx.doi.org/10.3390/molecules19033012 |
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author | Ni, Na Liu, Qiang Ren, Huixia Wu, Di Luo, Chuanming Li, Peng Wan, Jian-Bo Su, Huanxing |
author_facet | Ni, Na Liu, Qiang Ren, Huixia Wu, Di Luo, Chuanming Li, Peng Wan, Jian-Bo Su, Huanxing |
author_sort | Ni, Na |
collection | PubMed |
description | Ginseng, the root of Panax ginseng C.A. Meyer, has been used as a tonic to enhance bodily functions against various ailments for hundreds of years in Far Eastern countries without apparent adverse effects. Ginsenoside Rb1, one of the most active ingredients of ginseng, has been shown to possess various pharmacological activities. Here we report that Rb1 exhibits potent neuroprotective effects against oxidative injury induced by tert-butylhydroperoxide (t-BHP). Lactate dehydrogenase (LDH) assay demonstrated that incubation with 300 µm t-BHP for 2.5 h led to a significant cell loss of cultured rat embryonic cortex-derived neural progenitor cells (NPCs) and the cell viability was pronouncedly increased by 24 h pretreatment of 10 µm Rb1. TUNEL staining further confirmed that pretreatment of Rb1 significantly reduced the cell apoptosis in t-BHP-induced oxidative injury. Real time PCR revealed that pretreatment with Rb1 activated Nrf2 pathway in cultured NPCs and led to an elevated expression of HO-1. The results of the present study demonstrate that Rb1 shows a potent anti-oxidative effect on cultured NPCs by activating Nrf2 pathway. |
format | Online Article Text |
id | pubmed-6271345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62713452018-12-20 Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury Ni, Na Liu, Qiang Ren, Huixia Wu, Di Luo, Chuanming Li, Peng Wan, Jian-Bo Su, Huanxing Molecules Article Ginseng, the root of Panax ginseng C.A. Meyer, has been used as a tonic to enhance bodily functions against various ailments for hundreds of years in Far Eastern countries without apparent adverse effects. Ginsenoside Rb1, one of the most active ingredients of ginseng, has been shown to possess various pharmacological activities. Here we report that Rb1 exhibits potent neuroprotective effects against oxidative injury induced by tert-butylhydroperoxide (t-BHP). Lactate dehydrogenase (LDH) assay demonstrated that incubation with 300 µm t-BHP for 2.5 h led to a significant cell loss of cultured rat embryonic cortex-derived neural progenitor cells (NPCs) and the cell viability was pronouncedly increased by 24 h pretreatment of 10 µm Rb1. TUNEL staining further confirmed that pretreatment of Rb1 significantly reduced the cell apoptosis in t-BHP-induced oxidative injury. Real time PCR revealed that pretreatment with Rb1 activated Nrf2 pathway in cultured NPCs and led to an elevated expression of HO-1. The results of the present study demonstrate that Rb1 shows a potent anti-oxidative effect on cultured NPCs by activating Nrf2 pathway. MDPI 2014-03-07 /pmc/articles/PMC6271345/ /pubmed/24662068 http://dx.doi.org/10.3390/molecules19033012 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Ni, Na Liu, Qiang Ren, Huixia Wu, Di Luo, Chuanming Li, Peng Wan, Jian-Bo Su, Huanxing Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury |
title | Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury |
title_full | Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury |
title_fullStr | Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury |
title_full_unstemmed | Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury |
title_short | Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury |
title_sort | ginsenoside rb1 protects rat neural progenitor cells against oxidative injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271345/ https://www.ncbi.nlm.nih.gov/pubmed/24662068 http://dx.doi.org/10.3390/molecules19033012 |
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