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Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury

Ginseng, the root of Panax ginseng C.A. Meyer, has been used as a tonic to enhance bodily functions against various ailments for hundreds of years in Far Eastern countries without apparent adverse effects. Ginsenoside Rb1, one of the most active ingredients of ginseng, has been shown to possess vari...

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Autores principales: Ni, Na, Liu, Qiang, Ren, Huixia, Wu, Di, Luo, Chuanming, Li, Peng, Wan, Jian-Bo, Su, Huanxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271345/
https://www.ncbi.nlm.nih.gov/pubmed/24662068
http://dx.doi.org/10.3390/molecules19033012
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author Ni, Na
Liu, Qiang
Ren, Huixia
Wu, Di
Luo, Chuanming
Li, Peng
Wan, Jian-Bo
Su, Huanxing
author_facet Ni, Na
Liu, Qiang
Ren, Huixia
Wu, Di
Luo, Chuanming
Li, Peng
Wan, Jian-Bo
Su, Huanxing
author_sort Ni, Na
collection PubMed
description Ginseng, the root of Panax ginseng C.A. Meyer, has been used as a tonic to enhance bodily functions against various ailments for hundreds of years in Far Eastern countries without apparent adverse effects. Ginsenoside Rb1, one of the most active ingredients of ginseng, has been shown to possess various pharmacological activities. Here we report that Rb1 exhibits potent neuroprotective effects against oxidative injury induced by tert-butylhydroperoxide (t-BHP). Lactate dehydrogenase (LDH) assay demonstrated that incubation with 300 µm t-BHP for 2.5 h led to a significant cell loss of cultured rat embryonic cortex-derived neural progenitor cells (NPCs) and the cell viability was pronouncedly increased by 24 h pretreatment of 10 µm Rb1. TUNEL staining further confirmed that pretreatment of Rb1 significantly reduced the cell apoptosis in t-BHP-induced oxidative injury. Real time PCR revealed that pretreatment with Rb1 activated Nrf2 pathway in cultured NPCs and led to an elevated expression of HO-1. The results of the present study demonstrate that Rb1 shows a potent anti-oxidative effect on cultured NPCs by activating Nrf2 pathway.
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spelling pubmed-62713452018-12-20 Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury Ni, Na Liu, Qiang Ren, Huixia Wu, Di Luo, Chuanming Li, Peng Wan, Jian-Bo Su, Huanxing Molecules Article Ginseng, the root of Panax ginseng C.A. Meyer, has been used as a tonic to enhance bodily functions against various ailments for hundreds of years in Far Eastern countries without apparent adverse effects. Ginsenoside Rb1, one of the most active ingredients of ginseng, has been shown to possess various pharmacological activities. Here we report that Rb1 exhibits potent neuroprotective effects against oxidative injury induced by tert-butylhydroperoxide (t-BHP). Lactate dehydrogenase (LDH) assay demonstrated that incubation with 300 µm t-BHP for 2.5 h led to a significant cell loss of cultured rat embryonic cortex-derived neural progenitor cells (NPCs) and the cell viability was pronouncedly increased by 24 h pretreatment of 10 µm Rb1. TUNEL staining further confirmed that pretreatment of Rb1 significantly reduced the cell apoptosis in t-BHP-induced oxidative injury. Real time PCR revealed that pretreatment with Rb1 activated Nrf2 pathway in cultured NPCs and led to an elevated expression of HO-1. The results of the present study demonstrate that Rb1 shows a potent anti-oxidative effect on cultured NPCs by activating Nrf2 pathway. MDPI 2014-03-07 /pmc/articles/PMC6271345/ /pubmed/24662068 http://dx.doi.org/10.3390/molecules19033012 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ni, Na
Liu, Qiang
Ren, Huixia
Wu, Di
Luo, Chuanming
Li, Peng
Wan, Jian-Bo
Su, Huanxing
Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury
title Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury
title_full Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury
title_fullStr Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury
title_full_unstemmed Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury
title_short Ginsenoside Rb1 Protects Rat Neural Progenitor Cells against Oxidative Injury
title_sort ginsenoside rb1 protects rat neural progenitor cells against oxidative injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271345/
https://www.ncbi.nlm.nih.gov/pubmed/24662068
http://dx.doi.org/10.3390/molecules19033012
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