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Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems

Redox-modulating compounds derived from natural sources, such as redox active secondary metabolites, are currently of considerable interest in the field of chemoprevention, drug and phytoprotectant development. Unfortunately, the exact and occasionally even selective activity of such products, and t...

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Autores principales: Mániková, Dominika, Medvecová Letavayová, Lucia, Vlasáková, Danuša, Košík, Pavol, Castellucci Estevam, Ethiene, Nasim, Muhammad Jawad, Gruhlke, Martin, Slusarenko, Alan, Burkholz, Torsten, Jacob, Claus, Chovanec, Miroslav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271387/
https://www.ncbi.nlm.nih.gov/pubmed/25123189
http://dx.doi.org/10.3390/molecules190812258
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author Mániková, Dominika
Medvecová Letavayová, Lucia
Vlasáková, Danuša
Košík, Pavol
Castellucci Estevam, Ethiene
Nasim, Muhammad Jawad
Gruhlke, Martin
Slusarenko, Alan
Burkholz, Torsten
Jacob, Claus
Chovanec, Miroslav
author_facet Mániková, Dominika
Medvecová Letavayová, Lucia
Vlasáková, Danuša
Košík, Pavol
Castellucci Estevam, Ethiene
Nasim, Muhammad Jawad
Gruhlke, Martin
Slusarenko, Alan
Burkholz, Torsten
Jacob, Claus
Chovanec, Miroslav
author_sort Mániková, Dominika
collection PubMed
description Redox-modulating compounds derived from natural sources, such as redox active secondary metabolites, are currently of considerable interest in the field of chemoprevention, drug and phytoprotectant development. Unfortunately, the exact and occasionally even selective activity of such products, and the underlying (bio-)chemical causes thereof, are often only poorly understood. A combination of the nematode- and yeast-based assays provides a powerful platform to investigate a possible biological activity of a new compound and also to explore the “redox link” which may exist between its activity on the one side and its chemistry on the other. Here, we will demonstrate the usefulness of this platform for screening several selenium and tellurium compounds for their activity and action. We will also show how the nematode-based assay can be used to obtain information on compound uptake and distribution inside a multicellular organism, whilst the yeast-based system can be employed to explore possible intracellular mechanisms via chemogenetic screening and intracellular diagnostics. Whilst none of these simple and easy-to-use assays can ultimately substitute for in-depth studies in human cells and animals, these methods nonetheless provide a first glimpse on the possible biological activities of new compounds and offer direction for more complicated future investigations. They may also uncover some rather unpleasant biochemical actions of certain compounds, such as the ability of the trace element supplement selenite to induce DNA strand breaks.
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spelling pubmed-62713872018-12-27 Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems Mániková, Dominika Medvecová Letavayová, Lucia Vlasáková, Danuša Košík, Pavol Castellucci Estevam, Ethiene Nasim, Muhammad Jawad Gruhlke, Martin Slusarenko, Alan Burkholz, Torsten Jacob, Claus Chovanec, Miroslav Molecules Article Redox-modulating compounds derived from natural sources, such as redox active secondary metabolites, are currently of considerable interest in the field of chemoprevention, drug and phytoprotectant development. Unfortunately, the exact and occasionally even selective activity of such products, and the underlying (bio-)chemical causes thereof, are often only poorly understood. A combination of the nematode- and yeast-based assays provides a powerful platform to investigate a possible biological activity of a new compound and also to explore the “redox link” which may exist between its activity on the one side and its chemistry on the other. Here, we will demonstrate the usefulness of this platform for screening several selenium and tellurium compounds for their activity and action. We will also show how the nematode-based assay can be used to obtain information on compound uptake and distribution inside a multicellular organism, whilst the yeast-based system can be employed to explore possible intracellular mechanisms via chemogenetic screening and intracellular diagnostics. Whilst none of these simple and easy-to-use assays can ultimately substitute for in-depth studies in human cells and animals, these methods nonetheless provide a first glimpse on the possible biological activities of new compounds and offer direction for more complicated future investigations. They may also uncover some rather unpleasant biochemical actions of certain compounds, such as the ability of the trace element supplement selenite to induce DNA strand breaks. MDPI 2014-08-13 /pmc/articles/PMC6271387/ /pubmed/25123189 http://dx.doi.org/10.3390/molecules190812258 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Mániková, Dominika
Medvecová Letavayová, Lucia
Vlasáková, Danuša
Košík, Pavol
Castellucci Estevam, Ethiene
Nasim, Muhammad Jawad
Gruhlke, Martin
Slusarenko, Alan
Burkholz, Torsten
Jacob, Claus
Chovanec, Miroslav
Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems
title Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems
title_full Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems
title_fullStr Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems
title_full_unstemmed Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems
title_short Intracellular Diagnostics: Hunting for the Mode of Action of Redox-Modulating Selenium Compounds in Selected Model Systems
title_sort intracellular diagnostics: hunting for the mode of action of redox-modulating selenium compounds in selected model systems
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271387/
https://www.ncbi.nlm.nih.gov/pubmed/25123189
http://dx.doi.org/10.3390/molecules190812258
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