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Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling

This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of C...

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Autores principales: Lin, Chi Chen, Chen, Hua Han, Chen, Yu Kuo, Chang, Hung Chia, Lin, Ping Yi, Pan, I-Hong, Chen, Der-Yuan, Chen, Chuan Mu, Lin, Su Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271473/
https://www.ncbi.nlm.nih.gov/pubmed/24762969
http://dx.doi.org/10.3390/molecules19045325
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author Lin, Chi Chen
Chen, Hua Han
Chen, Yu Kuo
Chang, Hung Chia
Lin, Ping Yi
Pan, I-Hong
Chen, Der-Yuan
Chen, Chuan Mu
Lin, Su Yi
author_facet Lin, Chi Chen
Chen, Hua Han
Chen, Yu Kuo
Chang, Hung Chia
Lin, Ping Yi
Pan, I-Hong
Chen, Der-Yuan
Chen, Chuan Mu
Lin, Su Yi
author_sort Lin, Chi Chen
collection PubMed
description This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of CD40, CD80/CD86 and MHC-I/II molecules. FOs efficiently induced maturation of DCs generated from C3H/HeN or C57BL/6 mice with normal toll-like receptor 4 (TLR-4) or TLR-2 but not DCs from mice with mutated TLR4 or TLR2. The mechanism of action of FOs may be mediated by increased phosphorylation of ERK, p38 and JNK mitogen-activated protein kinase (MAPKs) and increased NF-κB activity, which are important signaling molecules downstream of TLR-4 and TLR-2. These data suggest that FOs induce DCs maturation through TLR-4 and/or TLR-2 and that FOs might have potential efficacy against tumor or virus infection or represent a candidate-adjuvant approach for application in immunotherapy and vaccination.
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spelling pubmed-62714732019-01-02 Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling Lin, Chi Chen Chen, Hua Han Chen, Yu Kuo Chang, Hung Chia Lin, Ping Yi Pan, I-Hong Chen, Der-Yuan Chen, Chuan Mu Lin, Su Yi Molecules Article This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of CD40, CD80/CD86 and MHC-I/II molecules. FOs efficiently induced maturation of DCs generated from C3H/HeN or C57BL/6 mice with normal toll-like receptor 4 (TLR-4) or TLR-2 but not DCs from mice with mutated TLR4 or TLR2. The mechanism of action of FOs may be mediated by increased phosphorylation of ERK, p38 and JNK mitogen-activated protein kinase (MAPKs) and increased NF-κB activity, which are important signaling molecules downstream of TLR-4 and TLR-2. These data suggest that FOs induce DCs maturation through TLR-4 and/or TLR-2 and that FOs might have potential efficacy against tumor or virus infection or represent a candidate-adjuvant approach for application in immunotherapy and vaccination. MDPI 2014-04-23 /pmc/articles/PMC6271473/ /pubmed/24762969 http://dx.doi.org/10.3390/molecules19045325 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Lin, Chi Chen
Chen, Hua Han
Chen, Yu Kuo
Chang, Hung Chia
Lin, Ping Yi
Pan, I-Hong
Chen, Der-Yuan
Chen, Chuan Mu
Lin, Su Yi
Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling
title Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling
title_full Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling
title_fullStr Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling
title_full_unstemmed Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling
title_short Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling
title_sort rice bran feruloylated oligosaccharides activate dendritic cells via toll-like receptor 2 and 4 signaling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271473/
https://www.ncbi.nlm.nih.gov/pubmed/24762969
http://dx.doi.org/10.3390/molecules19045325
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