Cargando…
Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling
This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of C...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271473/ https://www.ncbi.nlm.nih.gov/pubmed/24762969 http://dx.doi.org/10.3390/molecules19045325 |
_version_ | 1783376935840120832 |
---|---|
author | Lin, Chi Chen Chen, Hua Han Chen, Yu Kuo Chang, Hung Chia Lin, Ping Yi Pan, I-Hong Chen, Der-Yuan Chen, Chuan Mu Lin, Su Yi |
author_facet | Lin, Chi Chen Chen, Hua Han Chen, Yu Kuo Chang, Hung Chia Lin, Ping Yi Pan, I-Hong Chen, Der-Yuan Chen, Chuan Mu Lin, Su Yi |
author_sort | Lin, Chi Chen |
collection | PubMed |
description | This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of CD40, CD80/CD86 and MHC-I/II molecules. FOs efficiently induced maturation of DCs generated from C3H/HeN or C57BL/6 mice with normal toll-like receptor 4 (TLR-4) or TLR-2 but not DCs from mice with mutated TLR4 or TLR2. The mechanism of action of FOs may be mediated by increased phosphorylation of ERK, p38 and JNK mitogen-activated protein kinase (MAPKs) and increased NF-κB activity, which are important signaling molecules downstream of TLR-4 and TLR-2. These data suggest that FOs induce DCs maturation through TLR-4 and/or TLR-2 and that FOs might have potential efficacy against tumor or virus infection or represent a candidate-adjuvant approach for application in immunotherapy and vaccination. |
format | Online Article Text |
id | pubmed-6271473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62714732019-01-02 Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling Lin, Chi Chen Chen, Hua Han Chen, Yu Kuo Chang, Hung Chia Lin, Ping Yi Pan, I-Hong Chen, Der-Yuan Chen, Chuan Mu Lin, Su Yi Molecules Article This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of CD40, CD80/CD86 and MHC-I/II molecules. FOs efficiently induced maturation of DCs generated from C3H/HeN or C57BL/6 mice with normal toll-like receptor 4 (TLR-4) or TLR-2 but not DCs from mice with mutated TLR4 or TLR2. The mechanism of action of FOs may be mediated by increased phosphorylation of ERK, p38 and JNK mitogen-activated protein kinase (MAPKs) and increased NF-κB activity, which are important signaling molecules downstream of TLR-4 and TLR-2. These data suggest that FOs induce DCs maturation through TLR-4 and/or TLR-2 and that FOs might have potential efficacy against tumor or virus infection or represent a candidate-adjuvant approach for application in immunotherapy and vaccination. MDPI 2014-04-23 /pmc/articles/PMC6271473/ /pubmed/24762969 http://dx.doi.org/10.3390/molecules19045325 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Lin, Chi Chen Chen, Hua Han Chen, Yu Kuo Chang, Hung Chia Lin, Ping Yi Pan, I-Hong Chen, Der-Yuan Chen, Chuan Mu Lin, Su Yi Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling |
title | Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling |
title_full | Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling |
title_fullStr | Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling |
title_full_unstemmed | Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling |
title_short | Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling |
title_sort | rice bran feruloylated oligosaccharides activate dendritic cells via toll-like receptor 2 and 4 signaling |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271473/ https://www.ncbi.nlm.nih.gov/pubmed/24762969 http://dx.doi.org/10.3390/molecules19045325 |
work_keys_str_mv | AT linchichen ricebranferuloylatedoligosaccharidesactivatedendriticcellsviatolllikereceptor2and4signaling AT chenhuahan ricebranferuloylatedoligosaccharidesactivatedendriticcellsviatolllikereceptor2and4signaling AT chenyukuo ricebranferuloylatedoligosaccharidesactivatedendriticcellsviatolllikereceptor2and4signaling AT changhungchia ricebranferuloylatedoligosaccharidesactivatedendriticcellsviatolllikereceptor2and4signaling AT linpingyi ricebranferuloylatedoligosaccharidesactivatedendriticcellsviatolllikereceptor2and4signaling AT panihong ricebranferuloylatedoligosaccharidesactivatedendriticcellsviatolllikereceptor2and4signaling AT chenderyuan ricebranferuloylatedoligosaccharidesactivatedendriticcellsviatolllikereceptor2and4signaling AT chenchuanmu ricebranferuloylatedoligosaccharidesactivatedendriticcellsviatolllikereceptor2and4signaling AT linsuyi ricebranferuloylatedoligosaccharidesactivatedendriticcellsviatolllikereceptor2and4signaling |