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Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes
The aim was to provide the insight into the biology of C. albicans influenced by undescribed yet properties of saponin-rich (80%–98%) fractions (SAPFs), isolated from extracts of Trifolium alexandrinum, T. incarnatum, T. resupinatum var. resupinatum aerial parts. Their concentrations below 0.5 mg/mL...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271525/ https://www.ncbi.nlm.nih.gov/pubmed/25050858 http://dx.doi.org/10.3390/molecules190710601 |
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author | Budzyńska, Aleksandra Sadowska, Beata Więckowska-Szakiel, Marzena Micota, Bartłomiej Stochmal, Anna Jędrejek, Dariusz Pecio, Łukasz Różalska, Barbara |
author_facet | Budzyńska, Aleksandra Sadowska, Beata Więckowska-Szakiel, Marzena Micota, Bartłomiej Stochmal, Anna Jędrejek, Dariusz Pecio, Łukasz Różalska, Barbara |
author_sort | Budzyńska, Aleksandra |
collection | PubMed |
description | The aim was to provide the insight into the biology of C. albicans influenced by undescribed yet properties of saponin-rich (80%–98%) fractions (SAPFs), isolated from extracts of Trifolium alexandrinum, T. incarnatum, T. resupinatum var. resupinatum aerial parts. Their concentrations below 0.5 mg/mL were arbitrarily considered as subMICs for C. albicans ATCC 10231 and were further used. SAPFs affected yeast enzymatic activity, lowered tolerance to the oxidative stress, to the osmotic stress and to the action of the cell wall disrupting agent. In their presence, germ tubes formation was significantly and irreversibly inhibited, as well as Candida invasive capacity. The evaluation of SAPFs interactions with anti-mycotics showed synergistic activity, mainly with azoles. Fluconazole MIC was lowered—susceptible C. albicans ATCC 10231 was more susceptible, and resistant C. glabrata (clinical strain) become more susceptible (eightfold). Moreover, the tested samples showed no hemolytic activity and at the concentrations up to 0.5 mg/mL did not reduce viability of fibroblasts L929. This study provided the original evidence that SAPFs of Trifolium spp. aerial part exhibit significant antimicrobial activity, by reduce the expression/quantity of important Candida virulence factors and have good potential for the development of novel antifungal products supporting classic drugs. |
format | Online Article Text |
id | pubmed-6271525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62715252018-12-21 Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes Budzyńska, Aleksandra Sadowska, Beata Więckowska-Szakiel, Marzena Micota, Bartłomiej Stochmal, Anna Jędrejek, Dariusz Pecio, Łukasz Różalska, Barbara Molecules Article The aim was to provide the insight into the biology of C. albicans influenced by undescribed yet properties of saponin-rich (80%–98%) fractions (SAPFs), isolated from extracts of Trifolium alexandrinum, T. incarnatum, T. resupinatum var. resupinatum aerial parts. Their concentrations below 0.5 mg/mL were arbitrarily considered as subMICs for C. albicans ATCC 10231 and were further used. SAPFs affected yeast enzymatic activity, lowered tolerance to the oxidative stress, to the osmotic stress and to the action of the cell wall disrupting agent. In their presence, germ tubes formation was significantly and irreversibly inhibited, as well as Candida invasive capacity. The evaluation of SAPFs interactions with anti-mycotics showed synergistic activity, mainly with azoles. Fluconazole MIC was lowered—susceptible C. albicans ATCC 10231 was more susceptible, and resistant C. glabrata (clinical strain) become more susceptible (eightfold). Moreover, the tested samples showed no hemolytic activity and at the concentrations up to 0.5 mg/mL did not reduce viability of fibroblasts L929. This study provided the original evidence that SAPFs of Trifolium spp. aerial part exhibit significant antimicrobial activity, by reduce the expression/quantity of important Candida virulence factors and have good potential for the development of novel antifungal products supporting classic drugs. MDPI 2014-07-21 /pmc/articles/PMC6271525/ /pubmed/25050858 http://dx.doi.org/10.3390/molecules190710601 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Budzyńska, Aleksandra Sadowska, Beata Więckowska-Szakiel, Marzena Micota, Bartłomiej Stochmal, Anna Jędrejek, Dariusz Pecio, Łukasz Różalska, Barbara Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes |
title | Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes |
title_full | Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes |
title_fullStr | Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes |
title_full_unstemmed | Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes |
title_short | Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes |
title_sort | saponins of trifolium spp. aerial parts as modulators of candida albicans virulence attributes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271525/ https://www.ncbi.nlm.nih.gov/pubmed/25050858 http://dx.doi.org/10.3390/molecules190710601 |
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