Cargando…

Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes

The aim was to provide the insight into the biology of C. albicans influenced by undescribed yet properties of saponin-rich (80%–98%) fractions (SAPFs), isolated from extracts of Trifolium alexandrinum, T. incarnatum, T. resupinatum var. resupinatum aerial parts. Their concentrations below 0.5 mg/mL...

Descripción completa

Detalles Bibliográficos
Autores principales: Budzyńska, Aleksandra, Sadowska, Beata, Więckowska-Szakiel, Marzena, Micota, Bartłomiej, Stochmal, Anna, Jędrejek, Dariusz, Pecio, Łukasz, Różalska, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271525/
https://www.ncbi.nlm.nih.gov/pubmed/25050858
http://dx.doi.org/10.3390/molecules190710601
_version_ 1783376947808567296
author Budzyńska, Aleksandra
Sadowska, Beata
Więckowska-Szakiel, Marzena
Micota, Bartłomiej
Stochmal, Anna
Jędrejek, Dariusz
Pecio, Łukasz
Różalska, Barbara
author_facet Budzyńska, Aleksandra
Sadowska, Beata
Więckowska-Szakiel, Marzena
Micota, Bartłomiej
Stochmal, Anna
Jędrejek, Dariusz
Pecio, Łukasz
Różalska, Barbara
author_sort Budzyńska, Aleksandra
collection PubMed
description The aim was to provide the insight into the biology of C. albicans influenced by undescribed yet properties of saponin-rich (80%–98%) fractions (SAPFs), isolated from extracts of Trifolium alexandrinum, T. incarnatum, T. resupinatum var. resupinatum aerial parts. Their concentrations below 0.5 mg/mL were arbitrarily considered as subMICs for C. albicans ATCC 10231 and were further used. SAPFs affected yeast enzymatic activity, lowered tolerance to the oxidative stress, to the osmotic stress and to the action of the cell wall disrupting agent. In their presence, germ tubes formation was significantly and irreversibly inhibited, as well as Candida invasive capacity. The evaluation of SAPFs interactions with anti-mycotics showed synergistic activity, mainly with azoles. Fluconazole MIC was lowered—susceptible C. albicans ATCC 10231 was more susceptible, and resistant C. glabrata (clinical strain) become more susceptible (eightfold). Moreover, the tested samples showed no hemolytic activity and at the concentrations up to 0.5 mg/mL did not reduce viability of fibroblasts L929. This study provided the original evidence that SAPFs of Trifolium spp. aerial part exhibit significant antimicrobial activity, by reduce the expression/quantity of important Candida virulence factors and have good potential for the development of novel antifungal products supporting classic drugs.
format Online
Article
Text
id pubmed-6271525
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62715252018-12-21 Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes Budzyńska, Aleksandra Sadowska, Beata Więckowska-Szakiel, Marzena Micota, Bartłomiej Stochmal, Anna Jędrejek, Dariusz Pecio, Łukasz Różalska, Barbara Molecules Article The aim was to provide the insight into the biology of C. albicans influenced by undescribed yet properties of saponin-rich (80%–98%) fractions (SAPFs), isolated from extracts of Trifolium alexandrinum, T. incarnatum, T. resupinatum var. resupinatum aerial parts. Their concentrations below 0.5 mg/mL were arbitrarily considered as subMICs for C. albicans ATCC 10231 and were further used. SAPFs affected yeast enzymatic activity, lowered tolerance to the oxidative stress, to the osmotic stress and to the action of the cell wall disrupting agent. In their presence, germ tubes formation was significantly and irreversibly inhibited, as well as Candida invasive capacity. The evaluation of SAPFs interactions with anti-mycotics showed synergistic activity, mainly with azoles. Fluconazole MIC was lowered—susceptible C. albicans ATCC 10231 was more susceptible, and resistant C. glabrata (clinical strain) become more susceptible (eightfold). Moreover, the tested samples showed no hemolytic activity and at the concentrations up to 0.5 mg/mL did not reduce viability of fibroblasts L929. This study provided the original evidence that SAPFs of Trifolium spp. aerial part exhibit significant antimicrobial activity, by reduce the expression/quantity of important Candida virulence factors and have good potential for the development of novel antifungal products supporting classic drugs. MDPI 2014-07-21 /pmc/articles/PMC6271525/ /pubmed/25050858 http://dx.doi.org/10.3390/molecules190710601 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Budzyńska, Aleksandra
Sadowska, Beata
Więckowska-Szakiel, Marzena
Micota, Bartłomiej
Stochmal, Anna
Jędrejek, Dariusz
Pecio, Łukasz
Różalska, Barbara
Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes
title Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes
title_full Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes
title_fullStr Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes
title_full_unstemmed Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes
title_short Saponins of Trifolium spp. Aerial Parts as Modulators of Candida Albicans Virulence Attributes
title_sort saponins of trifolium spp. aerial parts as modulators of candida albicans virulence attributes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271525/
https://www.ncbi.nlm.nih.gov/pubmed/25050858
http://dx.doi.org/10.3390/molecules190710601
work_keys_str_mv AT budzynskaaleksandra saponinsoftrifoliumsppaerialpartsasmodulatorsofcandidaalbicansvirulenceattributes
AT sadowskabeata saponinsoftrifoliumsppaerialpartsasmodulatorsofcandidaalbicansvirulenceattributes
AT wieckowskaszakielmarzena saponinsoftrifoliumsppaerialpartsasmodulatorsofcandidaalbicansvirulenceattributes
AT micotabartłomiej saponinsoftrifoliumsppaerialpartsasmodulatorsofcandidaalbicansvirulenceattributes
AT stochmalanna saponinsoftrifoliumsppaerialpartsasmodulatorsofcandidaalbicansvirulenceattributes
AT jedrejekdariusz saponinsoftrifoliumsppaerialpartsasmodulatorsofcandidaalbicansvirulenceattributes
AT peciołukasz saponinsoftrifoliumsppaerialpartsasmodulatorsofcandidaalbicansvirulenceattributes
AT rozalskabarbara saponinsoftrifoliumsppaerialpartsasmodulatorsofcandidaalbicansvirulenceattributes