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Evaluation of Four Different Analytical Tools to Determine the Regional Origin of Gastrodia elata and Rehmannia glutinosa on the Basis of Metabolomics Study
Chemical profiles of medicinal plants could be dissimilar depending on the cultivation environments, which may influence their therapeutic efficacy. Accordingly, the regional origin of the medicinal plants should be authenticated for correct evaluation of their medicinal and market values. Metabolom...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271526/ https://www.ncbi.nlm.nih.gov/pubmed/24840900 http://dx.doi.org/10.3390/molecules19056294 |
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author | Lee, Dong-Kyu Lim, Dong Kyu Um, Jung A. Lim, Chang Ju Hong, Ji Yeon Yoon, Young A Ryu, Yeonsuk Kim, Hyo Jin Cho, Hi Jae Park, Jeong Hill Seo, Young Bae Kim, Kyunga Lim, Johan Kwon, Sung Won Lee, Jeongmi |
author_facet | Lee, Dong-Kyu Lim, Dong Kyu Um, Jung A. Lim, Chang Ju Hong, Ji Yeon Yoon, Young A Ryu, Yeonsuk Kim, Hyo Jin Cho, Hi Jae Park, Jeong Hill Seo, Young Bae Kim, Kyunga Lim, Johan Kwon, Sung Won Lee, Jeongmi |
author_sort | Lee, Dong-Kyu |
collection | PubMed |
description | Chemical profiles of medicinal plants could be dissimilar depending on the cultivation environments, which may influence their therapeutic efficacy. Accordingly, the regional origin of the medicinal plants should be authenticated for correct evaluation of their medicinal and market values. Metabolomics has been found very useful for discriminating the origin of many plants. Choosing the adequate analytical tool can be an essential procedure because different chemical profiles with different detection ranges will be produced according to the choice. In this study, four analytical tools, Fourier transform near‑infrared spectroscopy (FT-NIR), (1)H-nuclear magnetic resonance spectroscopy ((1)H‑NMR), liquid chromatography-mass spectrometry (LC-MS), and gas chromatography-mass spectroscopy (GC-MS) were applied in parallel to the same samples of two popular medicinal plants (Gastrodia elata and Rehmannia glutinosa) cultivated either in Korea or China. The classification abilities of four discriminant models for each plant were evaluated based on the misclassification rate and Q(2) obtained from principal component analysis (PCA) and orthogonal projection to latent structures-discriminant analysis (OPLS‑DA), respectively. (1)H-NMR and LC-MS, which were the best techniques for G. elata and R. glutinosa, respectively, were generally preferable for origin discrimination over the others. Reasoned by integrating all the results, (1)H-NMR is the most prominent technique for discriminating the origins of two plants. Nonetheless, this study suggests that preliminary screening is essential to determine the most suitable analytical tool and statistical method, which will ensure the dependability of metabolomics-based discrimination. |
format | Online Article Text |
id | pubmed-6271526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62715262018-12-21 Evaluation of Four Different Analytical Tools to Determine the Regional Origin of Gastrodia elata and Rehmannia glutinosa on the Basis of Metabolomics Study Lee, Dong-Kyu Lim, Dong Kyu Um, Jung A. Lim, Chang Ju Hong, Ji Yeon Yoon, Young A Ryu, Yeonsuk Kim, Hyo Jin Cho, Hi Jae Park, Jeong Hill Seo, Young Bae Kim, Kyunga Lim, Johan Kwon, Sung Won Lee, Jeongmi Molecules Article Chemical profiles of medicinal plants could be dissimilar depending on the cultivation environments, which may influence their therapeutic efficacy. Accordingly, the regional origin of the medicinal plants should be authenticated for correct evaluation of their medicinal and market values. Metabolomics has been found very useful for discriminating the origin of many plants. Choosing the adequate analytical tool can be an essential procedure because different chemical profiles with different detection ranges will be produced according to the choice. In this study, four analytical tools, Fourier transform near‑infrared spectroscopy (FT-NIR), (1)H-nuclear magnetic resonance spectroscopy ((1)H‑NMR), liquid chromatography-mass spectrometry (LC-MS), and gas chromatography-mass spectroscopy (GC-MS) were applied in parallel to the same samples of two popular medicinal plants (Gastrodia elata and Rehmannia glutinosa) cultivated either in Korea or China. The classification abilities of four discriminant models for each plant were evaluated based on the misclassification rate and Q(2) obtained from principal component analysis (PCA) and orthogonal projection to latent structures-discriminant analysis (OPLS‑DA), respectively. (1)H-NMR and LC-MS, which were the best techniques for G. elata and R. glutinosa, respectively, were generally preferable for origin discrimination over the others. Reasoned by integrating all the results, (1)H-NMR is the most prominent technique for discriminating the origins of two plants. Nonetheless, this study suggests that preliminary screening is essential to determine the most suitable analytical tool and statistical method, which will ensure the dependability of metabolomics-based discrimination. MDPI 2014-05-16 /pmc/articles/PMC6271526/ /pubmed/24840900 http://dx.doi.org/10.3390/molecules19056294 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Lee, Dong-Kyu Lim, Dong Kyu Um, Jung A. Lim, Chang Ju Hong, Ji Yeon Yoon, Young A Ryu, Yeonsuk Kim, Hyo Jin Cho, Hi Jae Park, Jeong Hill Seo, Young Bae Kim, Kyunga Lim, Johan Kwon, Sung Won Lee, Jeongmi Evaluation of Four Different Analytical Tools to Determine the Regional Origin of Gastrodia elata and Rehmannia glutinosa on the Basis of Metabolomics Study |
title | Evaluation of Four Different Analytical Tools to Determine the Regional Origin of Gastrodia elata and Rehmannia glutinosa on the Basis of Metabolomics Study |
title_full | Evaluation of Four Different Analytical Tools to Determine the Regional Origin of Gastrodia elata and Rehmannia glutinosa on the Basis of Metabolomics Study |
title_fullStr | Evaluation of Four Different Analytical Tools to Determine the Regional Origin of Gastrodia elata and Rehmannia glutinosa on the Basis of Metabolomics Study |
title_full_unstemmed | Evaluation of Four Different Analytical Tools to Determine the Regional Origin of Gastrodia elata and Rehmannia glutinosa on the Basis of Metabolomics Study |
title_short | Evaluation of Four Different Analytical Tools to Determine the Regional Origin of Gastrodia elata and Rehmannia glutinosa on the Basis of Metabolomics Study |
title_sort | evaluation of four different analytical tools to determine the regional origin of gastrodia elata and rehmannia glutinosa on the basis of metabolomics study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271526/ https://www.ncbi.nlm.nih.gov/pubmed/24840900 http://dx.doi.org/10.3390/molecules19056294 |
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