One-step affinity purification of fusion proteins with optimal monodispersity and biological activity: application to aggregation-prone HPV E6 proteins

BACKGROUND: Bacterial expression and purification of recombinant proteins under homogeneous active form is often challenging. Fusion to highly soluble carrier proteins such as Maltose Binding Protein (MBP) often improves their folding and solubility, but self-association may still occur. For instanc...

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Detalles Bibliográficos
Autores principales: Bonhoure, Anna, Demenge, Auguste, Kostmann, Camille, San José, Leticia, De la Cal, Eva, Armisen, Pilar, Nominé, Yves, Travé, Gilles
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271572/
https://www.ncbi.nlm.nih.gov/pubmed/30501645
http://dx.doi.org/10.1186/s12934-018-1039-z

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