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Specific cancer stem cell-therapy by albumin nanoparticles functionalized with CD44-mediated targeting
BACKGROUND: Cancer stem cells (CSCs) are highly proliferative and tumorigenic, which contributes to chemotherapy resistance and tumor occurrence. CSCs specific therapy may achieve excellent therapeutic effects, especially to the drug-resistant tumors. RESULTS: In this study, we developed a kind of t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271611/ https://www.ncbi.nlm.nih.gov/pubmed/30501644 http://dx.doi.org/10.1186/s12951-018-0424-4 |
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author | Li, Yuanyuan Shi, Sanjun Ming, Yue Wang, Linli Li, Chenwen Luo, Minghe Li, Ziwei Li, Bin Chen, Jianhong |
author_facet | Li, Yuanyuan Shi, Sanjun Ming, Yue Wang, Linli Li, Chenwen Luo, Minghe Li, Ziwei Li, Bin Chen, Jianhong |
author_sort | Li, Yuanyuan |
collection | PubMed |
description | BACKGROUND: Cancer stem cells (CSCs) are highly proliferative and tumorigenic, which contributes to chemotherapy resistance and tumor occurrence. CSCs specific therapy may achieve excellent therapeutic effects, especially to the drug-resistant tumors. RESULTS: In this study, we developed a kind of targeting nanoparticle system based on cationic albumin functionalized with hyaluronic acid (HA) to target the CD44 overexpressed CSCs. All-trans-retinoic acid (ATRA) was encapsulated in the nanoparticles with ultrahigh encapsulation efficiency (EE%) of 93% and loading content of 8.37%. TEM analysis showed the nanoparticles were spherical, uniform-sized and surrounded by a coating layer consists of HA. Four weeks of continuously measurements of size, PDI and EE% revealed the high stability of nanoparticles. Thanks to HA conjugation on the surface, the resultant nanoparticles (HA-eNPs) demonstrated high affinity and specific binding to CD44-enriched B16F10 cells. In vivo imaging revealed that HA-eNPs can targeted accumulate in tumor-bearing lung of mouse. The cytotoxicity tests illustrated that ATRA-laden HA-eNPs possessed better killing ability to B16F10 cells than free drug or normal nanoparticles in the same dose, indicating its good targeting property. Moreover, HA-eNPs/ATRA treatment decreased side population of B16F10 cells significantly in vitro. Finally, tumor growth was significantly inhibited by HA-eNPs/ATRA in lung metastasis tumor mice. CONCLUSIONS: These results demonstrate that the HA functionalized albumin nanoparticles is an efficient system for targeted delivery of antitumor drugs to eliminate the CSCs. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12951-018-0424-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6271611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-62716112018-12-05 Specific cancer stem cell-therapy by albumin nanoparticles functionalized with CD44-mediated targeting Li, Yuanyuan Shi, Sanjun Ming, Yue Wang, Linli Li, Chenwen Luo, Minghe Li, Ziwei Li, Bin Chen, Jianhong J Nanobiotechnology Research BACKGROUND: Cancer stem cells (CSCs) are highly proliferative and tumorigenic, which contributes to chemotherapy resistance and tumor occurrence. CSCs specific therapy may achieve excellent therapeutic effects, especially to the drug-resistant tumors. RESULTS: In this study, we developed a kind of targeting nanoparticle system based on cationic albumin functionalized with hyaluronic acid (HA) to target the CD44 overexpressed CSCs. All-trans-retinoic acid (ATRA) was encapsulated in the nanoparticles with ultrahigh encapsulation efficiency (EE%) of 93% and loading content of 8.37%. TEM analysis showed the nanoparticles were spherical, uniform-sized and surrounded by a coating layer consists of HA. Four weeks of continuously measurements of size, PDI and EE% revealed the high stability of nanoparticles. Thanks to HA conjugation on the surface, the resultant nanoparticles (HA-eNPs) demonstrated high affinity and specific binding to CD44-enriched B16F10 cells. In vivo imaging revealed that HA-eNPs can targeted accumulate in tumor-bearing lung of mouse. The cytotoxicity tests illustrated that ATRA-laden HA-eNPs possessed better killing ability to B16F10 cells than free drug or normal nanoparticles in the same dose, indicating its good targeting property. Moreover, HA-eNPs/ATRA treatment decreased side population of B16F10 cells significantly in vitro. Finally, tumor growth was significantly inhibited by HA-eNPs/ATRA in lung metastasis tumor mice. CONCLUSIONS: These results demonstrate that the HA functionalized albumin nanoparticles is an efficient system for targeted delivery of antitumor drugs to eliminate the CSCs. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12951-018-0424-4) contains supplementary material, which is available to authorized users. BioMed Central 2018-12-01 /pmc/articles/PMC6271611/ /pubmed/30501644 http://dx.doi.org/10.1186/s12951-018-0424-4 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Li, Yuanyuan Shi, Sanjun Ming, Yue Wang, Linli Li, Chenwen Luo, Minghe Li, Ziwei Li, Bin Chen, Jianhong Specific cancer stem cell-therapy by albumin nanoparticles functionalized with CD44-mediated targeting |
title | Specific cancer stem cell-therapy by albumin nanoparticles functionalized with CD44-mediated targeting |
title_full | Specific cancer stem cell-therapy by albumin nanoparticles functionalized with CD44-mediated targeting |
title_fullStr | Specific cancer stem cell-therapy by albumin nanoparticles functionalized with CD44-mediated targeting |
title_full_unstemmed | Specific cancer stem cell-therapy by albumin nanoparticles functionalized with CD44-mediated targeting |
title_short | Specific cancer stem cell-therapy by albumin nanoparticles functionalized with CD44-mediated targeting |
title_sort | specific cancer stem cell-therapy by albumin nanoparticles functionalized with cd44-mediated targeting |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271611/ https://www.ncbi.nlm.nih.gov/pubmed/30501644 http://dx.doi.org/10.1186/s12951-018-0424-4 |
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