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Unstable Simple Volatiles and Gas Chromatography-Tandem Mass Spectrometry Analysis of Essential Oil from the Roots Bark of Oplopanax Horridus Extracted by Supercritical Fluid Extraction

Volatile oil from the root bark of Oplopanax horridus is regarded to be responsible for the clinical uses of the title plant as a respiratory stimulant and expectorant. Therefore, a supercritical fluid extraction method was first employed to extract the volatile oil from the roots bark of O. horridu...

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Autores principales: Shao, Li, Bao, Mei-Hua, Ouyang, Dong-Sheng, Wang, Chong-Zhi, Yuan, Chun-Su, Zhou, Hong-Hao, Huang, Wei-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271617/
https://www.ncbi.nlm.nih.gov/pubmed/25438081
http://dx.doi.org/10.3390/molecules191219708
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author Shao, Li
Bao, Mei-Hua
Ouyang, Dong-Sheng
Wang, Chong-Zhi
Yuan, Chun-Su
Zhou, Hong-Hao
Huang, Wei-Hua
author_facet Shao, Li
Bao, Mei-Hua
Ouyang, Dong-Sheng
Wang, Chong-Zhi
Yuan, Chun-Su
Zhou, Hong-Hao
Huang, Wei-Hua
author_sort Shao, Li
collection PubMed
description Volatile oil from the root bark of Oplopanax horridus is regarded to be responsible for the clinical uses of the title plant as a respiratory stimulant and expectorant. Therefore, a supercritical fluid extraction method was first employed to extract the volatile oil from the roots bark of O. horridus, which was subsequently analyzed by GC/MS. Forty-eight volatile compounds were identified by GC/MS analysis, including (S,E)-nerolidol (52.5%), τ-cadinol (21.6%) and S-falcarinol (3.6%). Accordingly, the volatile oil (100 g) was subjected to chromatographic separation and purification. As a result, the three compounds, (E)-nerolidol (2 g), τ-cadinol (62 mg) and S-falcarinol (21 mg), were isolated and purified from the volatile oil, the structures of which were unambiguously elucidated by detailed spectroscopic analysis including 1D- and 2D-NMR techniques.
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spelling pubmed-62716172018-12-28 Unstable Simple Volatiles and Gas Chromatography-Tandem Mass Spectrometry Analysis of Essential Oil from the Roots Bark of Oplopanax Horridus Extracted by Supercritical Fluid Extraction Shao, Li Bao, Mei-Hua Ouyang, Dong-Sheng Wang, Chong-Zhi Yuan, Chun-Su Zhou, Hong-Hao Huang, Wei-Hua Molecules Article Volatile oil from the root bark of Oplopanax horridus is regarded to be responsible for the clinical uses of the title plant as a respiratory stimulant and expectorant. Therefore, a supercritical fluid extraction method was first employed to extract the volatile oil from the roots bark of O. horridus, which was subsequently analyzed by GC/MS. Forty-eight volatile compounds were identified by GC/MS analysis, including (S,E)-nerolidol (52.5%), τ-cadinol (21.6%) and S-falcarinol (3.6%). Accordingly, the volatile oil (100 g) was subjected to chromatographic separation and purification. As a result, the three compounds, (E)-nerolidol (2 g), τ-cadinol (62 mg) and S-falcarinol (21 mg), were isolated and purified from the volatile oil, the structures of which were unambiguously elucidated by detailed spectroscopic analysis including 1D- and 2D-NMR techniques. MDPI 2014-11-27 /pmc/articles/PMC6271617/ /pubmed/25438081 http://dx.doi.org/10.3390/molecules191219708 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shao, Li
Bao, Mei-Hua
Ouyang, Dong-Sheng
Wang, Chong-Zhi
Yuan, Chun-Su
Zhou, Hong-Hao
Huang, Wei-Hua
Unstable Simple Volatiles and Gas Chromatography-Tandem Mass Spectrometry Analysis of Essential Oil from the Roots Bark of Oplopanax Horridus Extracted by Supercritical Fluid Extraction
title Unstable Simple Volatiles and Gas Chromatography-Tandem Mass Spectrometry Analysis of Essential Oil from the Roots Bark of Oplopanax Horridus Extracted by Supercritical Fluid Extraction
title_full Unstable Simple Volatiles and Gas Chromatography-Tandem Mass Spectrometry Analysis of Essential Oil from the Roots Bark of Oplopanax Horridus Extracted by Supercritical Fluid Extraction
title_fullStr Unstable Simple Volatiles and Gas Chromatography-Tandem Mass Spectrometry Analysis of Essential Oil from the Roots Bark of Oplopanax Horridus Extracted by Supercritical Fluid Extraction
title_full_unstemmed Unstable Simple Volatiles and Gas Chromatography-Tandem Mass Spectrometry Analysis of Essential Oil from the Roots Bark of Oplopanax Horridus Extracted by Supercritical Fluid Extraction
title_short Unstable Simple Volatiles and Gas Chromatography-Tandem Mass Spectrometry Analysis of Essential Oil from the Roots Bark of Oplopanax Horridus Extracted by Supercritical Fluid Extraction
title_sort unstable simple volatiles and gas chromatography-tandem mass spectrometry analysis of essential oil from the roots bark of oplopanax horridus extracted by supercritical fluid extraction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271617/
https://www.ncbi.nlm.nih.gov/pubmed/25438081
http://dx.doi.org/10.3390/molecules191219708
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