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Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds
A reproducible analytical method using reverse-phase high liquid performance chromatography combined with UV detecting was developed for the quantitative determination of four compounds isolated from the ethanol extract of Phaseolus angularis seeds (PASE): oleanolic acid (1), oleanolic acid acetate...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271774/ https://www.ncbi.nlm.nih.gov/pubmed/25033058 http://dx.doi.org/10.3390/molecules190710309 |
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author | Hwang, Joo Tae Oh, Hyun-Mee Kim, Mi-Hwa Jeong, Hyung Jae Lee, Chul Jang, Hyun-Jae Lee, Seung Woong Park, Chan Sun Rho, Mun-Chual |
author_facet | Hwang, Joo Tae Oh, Hyun-Mee Kim, Mi-Hwa Jeong, Hyung Jae Lee, Chul Jang, Hyun-Jae Lee, Seung Woong Park, Chan Sun Rho, Mun-Chual |
author_sort | Hwang, Joo Tae |
collection | PubMed |
description | A reproducible analytical method using reverse-phase high liquid performance chromatography combined with UV detecting was developed for the quantitative determination of four compounds isolated from the ethanol extract of Phaseolus angularis seeds (PASE): oleanolic acid (1), oleanolic acid acetate (2), stigmasterol (3) and β-sitosterol (4). This method was fully validated in terms of linearity (r(2) > 0.999), accuracy (98.5%–100.8%), precision (<0.92%), LOD (<0.0035 mg/mL), and LOQ (<0.0115 mg/mL). The effects of the PASE and isolated compounds 1–4 on TLR4 activation were tested in THP1-Blue cells. Among the tested substances, compound 2 showed potent inhibitory activity with an IC(50) value of 3.89 ± 0.17 µM. |
format | Online Article Text |
id | pubmed-6271774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62717742018-12-21 Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds Hwang, Joo Tae Oh, Hyun-Mee Kim, Mi-Hwa Jeong, Hyung Jae Lee, Chul Jang, Hyun-Jae Lee, Seung Woong Park, Chan Sun Rho, Mun-Chual Molecules Article A reproducible analytical method using reverse-phase high liquid performance chromatography combined with UV detecting was developed for the quantitative determination of four compounds isolated from the ethanol extract of Phaseolus angularis seeds (PASE): oleanolic acid (1), oleanolic acid acetate (2), stigmasterol (3) and β-sitosterol (4). This method was fully validated in terms of linearity (r(2) > 0.999), accuracy (98.5%–100.8%), precision (<0.92%), LOD (<0.0035 mg/mL), and LOQ (<0.0115 mg/mL). The effects of the PASE and isolated compounds 1–4 on TLR4 activation were tested in THP1-Blue cells. Among the tested substances, compound 2 showed potent inhibitory activity with an IC(50) value of 3.89 ± 0.17 µM. MDPI 2014-07-16 /pmc/articles/PMC6271774/ /pubmed/25033058 http://dx.doi.org/10.3390/molecules190710309 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hwang, Joo Tae Oh, Hyun-Mee Kim, Mi-Hwa Jeong, Hyung Jae Lee, Chul Jang, Hyun-Jae Lee, Seung Woong Park, Chan Sun Rho, Mun-Chual Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds |
title | Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds |
title_full | Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds |
title_fullStr | Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds |
title_full_unstemmed | Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds |
title_short | Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds |
title_sort | simultaneous quantitation and validation of triterpenoids and phytosteroids in phaseolus angularis seeds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271774/ https://www.ncbi.nlm.nih.gov/pubmed/25033058 http://dx.doi.org/10.3390/molecules190710309 |
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