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Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production
The biofloculant production potential of a consortium of Streptomyces and Brachybacterium species were evaluated. Optimum bioflocculant yields (g/L) and flocculation activities (%) were observed for the following preferred nutritional sources: glucose (56%; 2.78 ± 0.15 g/L), (NH(4))(2)NO(3) (53%; 2....
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271801/ https://www.ncbi.nlm.nih.gov/pubmed/25076145 http://dx.doi.org/10.3390/molecules190811131 |
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author | Nwodo, Uchechukwu U. Okoh, Anthony I. |
author_facet | Nwodo, Uchechukwu U. Okoh, Anthony I. |
author_sort | Nwodo, Uchechukwu U. |
collection | PubMed |
description | The biofloculant production potential of a consortium of Streptomyces and Brachybacterium species were evaluated. Optimum bioflocculant yields (g/L) and flocculation activities (%) were observed for the following preferred nutritional sources: glucose (56%; 2.78 ± 0.15 g/L), (NH(4))(2)NO(3) (53%; 2.81 ± 0.37 g/L) and CaSO(4)·H(2)O (47%; 2.19 ± 0.13 g/L). A Plackett-Burman design revealed the critical fermentation media components. The concentrations of these components were optimized [glucose; 16.0, (NH(4))(2)NO(3); 0.5 and CaSO(4)·H(2)O; 1.2 (g/L)] through a central composite design with optimum bioflocculant yield of 3.02 g/L and flocculation activity of 63.7%. The regression coefficient (R(2) = 0.6569) indicates a weak estimation of the model’s adequacy and a high lack-of-fit value (34.1%). Lack of synergy in the consortium may have been responsible for the model inadequacy observed. FTIR spectrometry showed the bioflocculant to be a heteropolysaccharide, while SEM imaging revealed an amorphous loosely arranged fluffy structure with interstial spacing of less than 1 µm. |
format | Online Article Text |
id | pubmed-6271801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62718012018-12-27 Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production Nwodo, Uchechukwu U. Okoh, Anthony I. Molecules Article The biofloculant production potential of a consortium of Streptomyces and Brachybacterium species were evaluated. Optimum bioflocculant yields (g/L) and flocculation activities (%) were observed for the following preferred nutritional sources: glucose (56%; 2.78 ± 0.15 g/L), (NH(4))(2)NO(3) (53%; 2.81 ± 0.37 g/L) and CaSO(4)·H(2)O (47%; 2.19 ± 0.13 g/L). A Plackett-Burman design revealed the critical fermentation media components. The concentrations of these components were optimized [glucose; 16.0, (NH(4))(2)NO(3); 0.5 and CaSO(4)·H(2)O; 1.2 (g/L)] through a central composite design with optimum bioflocculant yield of 3.02 g/L and flocculation activity of 63.7%. The regression coefficient (R(2) = 0.6569) indicates a weak estimation of the model’s adequacy and a high lack-of-fit value (34.1%). Lack of synergy in the consortium may have been responsible for the model inadequacy observed. FTIR spectrometry showed the bioflocculant to be a heteropolysaccharide, while SEM imaging revealed an amorphous loosely arranged fluffy structure with interstial spacing of less than 1 µm. MDPI 2014-07-29 /pmc/articles/PMC6271801/ /pubmed/25076145 http://dx.doi.org/10.3390/molecules190811131 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Nwodo, Uchechukwu U. Okoh, Anthony I. Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production |
title | Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production |
title_full | Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production |
title_fullStr | Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production |
title_full_unstemmed | Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production |
title_short | Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production |
title_sort | mixed culture fermentation and media optimization by response surface model: streptomyces and brachybacterium species in bioflocculant production |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271801/ https://www.ncbi.nlm.nih.gov/pubmed/25076145 http://dx.doi.org/10.3390/molecules190811131 |
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