Cargando…
Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract
This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydraz...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271915/ https://www.ncbi.nlm.nih.gov/pubmed/25225720 http://dx.doi.org/10.3390/molecules190914567 |
_version_ | 1783377037643218944 |
---|---|
author | Sharma, Neelesh Samarakoon, Kalpa W. Gyawali, Rajendra Park, Yang-Ho Lee, Sung-Jin Oh, Sung Jong Lee, Tae-Hoon Jeong, Dong Kee |
author_facet | Sharma, Neelesh Samarakoon, Kalpa W. Gyawali, Rajendra Park, Yang-Ho Lee, Sung-Jin Oh, Sung Jong Lee, Tae-Hoon Jeong, Dong Kee |
author_sort | Sharma, Neelesh |
collection | PubMed |
description | This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl, and alkyl radical levels and by using an online high-performance liquid chromatography (HPLC)-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The E. hirta ethanol extract (0.5 mg/mL) exhibited DPPH-scavenging activity of 61.19% ± 0.22%, while the positive control (0.5 mg/mL ascorbic acid) had 100% ± 0.22% activity. The concentration of the extract required to trap 50% of DPPH (IC(50)) was 0.205 mg/mL. Online HPLC analysis of the extract also showed strong antioxidant activity. The anti-inflammatory activity of the E. hirta extract was assessed in lipopolysaccharide-induced RAW 264.7 macrophages. The anti-inflammatory activity was highest in the presence of 200 µg/mL E. hirta extract, and nitric oxide production was decreased significantly (p < 0.05). The extract also showed selective anticancer activity at a concentration of 100 µg/mL (p < 0.05). These results indicated that E. hirta may warrant further investigation for the development of antioxidant, anti-inflammatory, and anticancer herbal medications. |
format | Online Article Text |
id | pubmed-6271915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62719152018-12-27 Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract Sharma, Neelesh Samarakoon, Kalpa W. Gyawali, Rajendra Park, Yang-Ho Lee, Sung-Jin Oh, Sung Jong Lee, Tae-Hoon Jeong, Dong Kee Molecules Article This study evaluated the chemical composition, antioxidant, anti-inflammatory and anticancer activities of a Euphorbia hirta L. extract. The antioxidant activities of whole E. hirta ethanol extract were determined by electron spin resonance spectrophotometric analysis of 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl, and alkyl radical levels and by using an online high-performance liquid chromatography (HPLC)-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay. The E. hirta ethanol extract (0.5 mg/mL) exhibited DPPH-scavenging activity of 61.19% ± 0.22%, while the positive control (0.5 mg/mL ascorbic acid) had 100% ± 0.22% activity. The concentration of the extract required to trap 50% of DPPH (IC(50)) was 0.205 mg/mL. Online HPLC analysis of the extract also showed strong antioxidant activity. The anti-inflammatory activity of the E. hirta extract was assessed in lipopolysaccharide-induced RAW 264.7 macrophages. The anti-inflammatory activity was highest in the presence of 200 µg/mL E. hirta extract, and nitric oxide production was decreased significantly (p < 0.05). The extract also showed selective anticancer activity at a concentration of 100 µg/mL (p < 0.05). These results indicated that E. hirta may warrant further investigation for the development of antioxidant, anti-inflammatory, and anticancer herbal medications. MDPI 2014-09-15 /pmc/articles/PMC6271915/ /pubmed/25225720 http://dx.doi.org/10.3390/molecules190914567 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Sharma, Neelesh Samarakoon, Kalpa W. Gyawali, Rajendra Park, Yang-Ho Lee, Sung-Jin Oh, Sung Jong Lee, Tae-Hoon Jeong, Dong Kee Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
title | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
title_full | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
title_fullStr | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
title_full_unstemmed | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
title_short | Evaluation of the Antioxidant, Anti-Inflammatory, and Anticancer Activities of Euphorbia hirta Ethanolic Extract |
title_sort | evaluation of the antioxidant, anti-inflammatory, and anticancer activities of euphorbia hirta ethanolic extract |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271915/ https://www.ncbi.nlm.nih.gov/pubmed/25225720 http://dx.doi.org/10.3390/molecules190914567 |
work_keys_str_mv | AT sharmaneelesh evaluationoftheantioxidantantiinflammatoryandanticanceractivitiesofeuphorbiahirtaethanolicextract AT samarakoonkalpaw evaluationoftheantioxidantantiinflammatoryandanticanceractivitiesofeuphorbiahirtaethanolicextract AT gyawalirajendra evaluationoftheantioxidantantiinflammatoryandanticanceractivitiesofeuphorbiahirtaethanolicextract AT parkyangho evaluationoftheantioxidantantiinflammatoryandanticanceractivitiesofeuphorbiahirtaethanolicextract AT leesungjin evaluationoftheantioxidantantiinflammatoryandanticanceractivitiesofeuphorbiahirtaethanolicextract AT ohsungjong evaluationoftheantioxidantantiinflammatoryandanticanceractivitiesofeuphorbiahirtaethanolicextract AT leetaehoon evaluationoftheantioxidantantiinflammatoryandanticanceractivitiesofeuphorbiahirtaethanolicextract AT jeongdongkee evaluationoftheantioxidantantiinflammatoryandanticanceractivitiesofeuphorbiahirtaethanolicextract |