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MALDI-TOF MS Analysis of Proanthocyanidins in Two Lowland Tropical Forest Species of Cecropia: A First Look at Their Chemical Structures

The structural chemistry of proanthocyanidin molecules has been investigated in temperate zone plants, but few studies have been done with plants of the Amazonian lowland tropical wet forests where herbivore pressure is more extensive and diverse. Using MALDI-TOF mass spectrometry, we report unique...

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Autores principales: Huynh, Alex Van, Bevington, John M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271936/
https://www.ncbi.nlm.nih.gov/pubmed/25221870
http://dx.doi.org/10.3390/molecules190914484
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author Huynh, Alex Van
Bevington, John M.
author_facet Huynh, Alex Van
Bevington, John M.
author_sort Huynh, Alex Van
collection PubMed
description The structural chemistry of proanthocyanidin molecules has been investigated in temperate zone plants, but few studies have been done with plants of the Amazonian lowland tropical wet forests where herbivore pressure is more extensive and diverse. Using MALDI-TOF mass spectrometry, we report unique properties of the proanthocyanidin structural chemistry in two neotropical Cecropia species, C. polystachya, a myrmecophyte with mutualistic ants, and C. sciadophylla, a non-myrmecophyte lacking mutualistic ants. Our preliminary data suggests the presence of reportedly uncommon propelargonidin subunits in a majority of proanthocyanidin oligomers. The presence of 3-O-gallate proanthocyanidin monomers was also detected in the mass spectra of both species. Unlike other studies that have examined species growing at higher latitudes, oligomers composed of procyanidin, propelargonidin, and their 3-O-gallates were present in both Cecropia species while the presence of oligomers containing prodelphinidin units were absent or at lower levels. These distinctive features may suggest that proanthocyanidins in some tropical plant species may be an untapped source of proanthocyanidin structural complexity that warrants further investigation. Several differences between spectra of the two Cecropia species could also point to the presence of anti-herbivore defense tradeoffs between chemical defense quality and biotic defense between the two species.
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spelling pubmed-62719362018-12-27 MALDI-TOF MS Analysis of Proanthocyanidins in Two Lowland Tropical Forest Species of Cecropia: A First Look at Their Chemical Structures Huynh, Alex Van Bevington, John M. Molecules Communication The structural chemistry of proanthocyanidin molecules has been investigated in temperate zone plants, but few studies have been done with plants of the Amazonian lowland tropical wet forests where herbivore pressure is more extensive and diverse. Using MALDI-TOF mass spectrometry, we report unique properties of the proanthocyanidin structural chemistry in two neotropical Cecropia species, C. polystachya, a myrmecophyte with mutualistic ants, and C. sciadophylla, a non-myrmecophyte lacking mutualistic ants. Our preliminary data suggests the presence of reportedly uncommon propelargonidin subunits in a majority of proanthocyanidin oligomers. The presence of 3-O-gallate proanthocyanidin monomers was also detected in the mass spectra of both species. Unlike other studies that have examined species growing at higher latitudes, oligomers composed of procyanidin, propelargonidin, and their 3-O-gallates were present in both Cecropia species while the presence of oligomers containing prodelphinidin units were absent or at lower levels. These distinctive features may suggest that proanthocyanidins in some tropical plant species may be an untapped source of proanthocyanidin structural complexity that warrants further investigation. Several differences between spectra of the two Cecropia species could also point to the presence of anti-herbivore defense tradeoffs between chemical defense quality and biotic defense between the two species. MDPI 2014-09-12 /pmc/articles/PMC6271936/ /pubmed/25221870 http://dx.doi.org/10.3390/molecules190914484 Text en © 2014 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Huynh, Alex Van
Bevington, John M.
MALDI-TOF MS Analysis of Proanthocyanidins in Two Lowland Tropical Forest Species of Cecropia: A First Look at Their Chemical Structures
title MALDI-TOF MS Analysis of Proanthocyanidins in Two Lowland Tropical Forest Species of Cecropia: A First Look at Their Chemical Structures
title_full MALDI-TOF MS Analysis of Proanthocyanidins in Two Lowland Tropical Forest Species of Cecropia: A First Look at Their Chemical Structures
title_fullStr MALDI-TOF MS Analysis of Proanthocyanidins in Two Lowland Tropical Forest Species of Cecropia: A First Look at Their Chemical Structures
title_full_unstemmed MALDI-TOF MS Analysis of Proanthocyanidins in Two Lowland Tropical Forest Species of Cecropia: A First Look at Their Chemical Structures
title_short MALDI-TOF MS Analysis of Proanthocyanidins in Two Lowland Tropical Forest Species of Cecropia: A First Look at Their Chemical Structures
title_sort maldi-tof ms analysis of proanthocyanidins in two lowland tropical forest species of cecropia: a first look at their chemical structures
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271936/
https://www.ncbi.nlm.nih.gov/pubmed/25221870
http://dx.doi.org/10.3390/molecules190914484
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