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Application of the Cre/loxP Site-Specific Recombination System for Gene Transformation in Aurantiochytrium limacinum
The Cre/loxP site-specific recombination system was applied to Aurantiochytrium limacinum to obtain a transformant without the antibiotic resistance marker gene. First, the enhanced green fluorescent protein gene (egfp) and chloramphenicol resistance gene (Cm(r)), along with the two loxP loci, were...
Autores principales: | Sun, Hengyi, Chen, Hao, Zang, Xiaonan, Hou, Pan, Zhou, Bingbing, Liu, Yuantao, Wu, Fei, Cao, Xiaofei, Zhang, Xuecheng |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272215/ https://www.ncbi.nlm.nih.gov/pubmed/26039334 http://dx.doi.org/10.3390/molecules200610110 |
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